High Glucose Aggravates the Detrimental Effects of Pancreatic Stellate Cells on Beta-Cell Function

Figure 5

PSCs-CM increases INS-1 cell apoptosis in vitro. The apoptosis of INS-1 cells was detected by Annexin V-FITC/PI apoptosis detection kit. Both PSCs-CM and HG alone caused marked and significant increases in INS-1 cell apoptosis, and the effects of both treatments on apoptosis were additive, with the combination of PSCs-CM and HG exerting a significantly more detrimental effect than either treatment alone.