Alterations in the Corneal Nerve and Stem/Progenitor Cells in Diabetes: Preventive Effects of Insulin-Like Growth Factor-1 Treatment
Figure 1
Ocular properties in ob/ob mice. Representative histological photomicrographs of hematoxylin and eosin-stained globe sections that were obtained from normal and 12-week-old diabetic mice. Swelling and stratification of the diabetic corneal epithelium are noted in comparison with the normal cornea. Magnification: 200x. Representative biomicroscopy of a normal cornea ((a) and (c)) and of a diabetic mouse cornea after 12 weeks ((b) and (d)). Representative histological photomicrographs of corneal nerves that were immunostained with an anti-β-III-tubulin fluorescein isothiocyanate-conjugated antibody showing the epithelial nerve (left column), subbasal nerve plexus (middle column), and stromal nerves (right column) in the normal eye ((e), (f), and (g)) and in a 12-week-old diabetic mouse eye ((h), (i), and (j)). Note the development of marked nerve degeneration after 12 weeks when the nerves appear faint and interrupted. Magnification: 200x.
