Research Article

ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level

Figure 2

Immunofluorescent confocal microscopic analysis of ATPase-B1 expression in primary hepatocytes. Freshly isolated primary hepatocytes grown on cover slips were pretreated with Ad-ATPase-B1 (b) and Ad-GFP (c) at 30 MOIs for 48 h. After washing with PBS, the cells were immunostained with antibody against V5-tag and AlexaFluor 596-conjugated goat anti-mouse IgG and analyzed by confocal microscope.
356432.fig.002a
(a) Blank
356432.fig.002b
(b) Ad-ATPase-B1
356432.fig.002c
(c) Ad-GFP