ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level
Figure 3
Multiplicity of infection (MOI) test in HepG2 cells and primary hepatocytes. HepG2 cells (a) and the isolated primary hepatocytes were pretreated with Ad-ATPase-B1 and Ad-GFP with different MOIs, and ATPase-B1 mRNA (b) and protein ((c) A, ATPase-B1 expression in WT primary hepatocytes; B, ATPase-B1 expression in SR-BI primary hepatocytes; C, the internal control of GADPH in WT and SR-BI primary hepatocytes) levels were measured. In HepG2 cells and WT hepatocytes, mRNA expression increased with increased MOI, while mRNA expression peaked at 30 MOIs in SR-BI mouse.
