Menin interacts with FBP1. (a) Menin was immunoprecipitated from HeLa nuclear extract using either SQVb antibody (lanes 1 and 2) or H-MEN1b antibody (lanes 3 and 4). An antibody not related to menin (lanes 5 and 6) was included as a negative control. The arrows and black circles indicate ASH2, LEDGF, MEN1, FBP1, and RBBP5. (b) Menin (goat antibody from Bethyl) was immunoprecipitated from whole cell extracts prepared from HEK293 cells transfected with menin and FBP1 plasmids. Immunoblot analysis was performed to detect menin and FBP1. IgG was included as a negative control. (c) (upper panel) Coomassie blue staining of Flag-BAP and Flag-menin immunoprecipitates after production and purification from bacterial cells. (lower panel) Equal concentrations of Flag-menin and Flag-BAP were used to precipitate FBP1 from HEK293 cells transfected with FBP1 plasmid. Immunoblot analysis was performed for FBP1.
