Influence of Vitamin D Binding Protein on Accuracy of 25-Hydroxyvitamin D Measurement Using the ADVIA Centaur Vitamin D Total Assay

Figure 1

Bias and percent bias between the 25(OH)D results of the ADVIA Centaur Vitamin D Total assay and the LC-MS/MS method as a function of DBP concentration in healthy human serum pooled samples (endogenous and endogenous + spiked) (a, b, c, d), pregnancy (third trimester) samples (a, b, e, f), and renal dialysis samples (a, b, g, h). The bias ±1.96 standard deviation (SD) represents the 95% limits of agreement. To convert 25(OH)D concentrations to nanomoles per liter (nmol/L), multiply by 2.5.

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