The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro

Figure 10

Effects of treatment with 10⁻⁵ M 7-methoxycoumarin or osthole or neither (control) on calcified nodules formation in rat calverial osteoblasts (ROB). Alizarin Red-S stained calcified nodules formed at 12th day after osteogenic induction culture (a). Number (b), total area (c), and staining intensity (integrated optical density or IOD) (d) of calcified nodules were measured by Image-Pro Plus 6.0 software. Results are shown as the mean ± SD of triplicate cultures. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus 7-methoxycoumarin.

(a)

(b)

(c)

(d)