The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro
Figure 7
Time-course changes in the secretion of osteogenesis-related proteins after different days of osteogenic induction culture of rat bone marrow stromal cells (rBMSCs) treated with 10−5 M 7-methoxycoumarin or osthole or neither (control). Protein levels in conditioned medium were determined by ELISA assays for collagen-I (a), BMP-2 (b), osteocalcin (c), and OPN (d). Results were represented by three experiments and shown as the mean ± SD of triplicate wells. , versus control; , versus 7-methoxycoumarin.