Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis
Figure 1
(a) Adiponectin mRNA expression determined by quantitative real-time PCR in porcine corpora lutea, granulosa cells, and theca interna cells harvested on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. The results are expressed as means ± S.E.M. (). Bars with different letters at the top are different (). (b) Adiponectin protein expression determined by Western blotting analysis in porcine corpora lutea, granulosa, and theca interna cells on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. Upper panel: representative immunoblots (MM—molecular marker, G—granulosa cells, Th—theca interna cells); lower panel: densitometric analysis of adiponectin protein relative to actin protein. The noted values are expressed as means ± S.E.M. in arbitrary optical density units (). Bars with different letters at the top are different ().
