Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation

Figure 2

Prolactin family genes are upregulated in adipocytes derived from Men1-KO mESCs. (a) Schematic diagram comparing the mouse prolactin gene family locus with the syntenic human locus. The mouse prolactin gene family locus on chromosome 13 is shown on top and the syntenic human locus on chromosome 6 is shown at the bottom. Red, genes not differentially expressed in Men1-KO versus WT adipocytes or that did not yield a PCR product. Green, genes with the highest fold change (>5-fold) in Men1-KO versus WT adipocytes. Yellow highlight, genes present in both mouse and human. Arrows indicate the orientation of the coding strand. Chromosomal location in megabases (Mb) is from the UCSC genome browser using mouse genome version mm9 and human genome version hg19. (b) Relative expression of prolactin family genes. Prolactin family genes () were analyzed by QPCR using RNA isolated from adipocytes derived by in vitro differentiation of WT or Men1-KO mESCs. Microarray data and QPCR relative fold change (normalized to Gapdh) are shown. “No probe” indicates genes not present on the microarray, and “no amp” indicates genes that did not yield a PCR product.

(a)

(b)