Anaplastic Thyroid Carcinoma: A ceRNA Analysis Pointed to a Crosstalk between SOX2, TP53, and microRNA Biogenesis

Table 8

Example of REST analysis on SOX2 and SOX2 ceRNAs levels of transcription quantified by RT-PCR compared to controls and normalized against -actin expression in SW1736 ATC cell line after SOX2 3′UTR overexpression. P(H1) is the probability of the alternative hypothesis that the difference between sample and control groups is due only to chance.

Gene	Reaction efficiency	Expression	Std. error	95% C.I.	P(H1)	Result
SOX2	0.655	1.414	1.176–1.710	1.102–1.818	0.000	UP
DICER1	0.7025	1.207	0.952–1.536	0.912–1.600	0.680
TP53	0.77	1.105	0.918–1.332	0.881–1.386	0.661
RNASEN	0.75	1.039	0.889–1.222	0.833–1.299	0.830
EIF2C2	0.765	1.508	1.393–1.641	1.314–1.733	0.169	UP
COX8A	0.785	0.879	0.733–1.068	0.669–1.160	0.680
CCND1	0.7425	1.226	1.045–1.462	0.937–1.611	0.341
SOX2 3′UTR	0.64	3.092	2.577–3.762	2.330–4.119	0.000	UP