Research Article
Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts
Figure 3
Representative Western blot (a) and quantification (b) of β-catenin levels in the enriched nuclear fraction of rat osteoblast-like cells exposed to ghrelin (AG, 10−10 M for 1 and 2 h) alone or together with D-Lys3-GHRP-6 (D, 10−7 M 30 min before AG), a GHSR-1a receptor antagonist. β-catenin levels are normalized on β-actin protein levels and results are expressed as the ratio of β-catenin amount measured in treated cells to the amount obtained in untreated cells. Data are the mean ± SEM of five experiments versus untreated; versus 2 h AG treated cells.
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