Research Article
Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts
Figure 5
Effect of pretreatment with (a) D-Lys3-GHRP-6 (D, 10−7 M, 30 min before AG) or (b) the PKA inhibitor H89 ( M, 30 min before AG) on the stimulatory action of ghrelin (AG, 10−10 M) on rat osteoblast-like cells (rOB) viability. Cell viability was measured by MTT assay. Data are expressed as the percentage relative to control and are the means ± SEM of four replicates within a single experiment that was repeated at least three times. versus untreated cells; versus AG.
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