Research Article
Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts
Figure 6
Effect of increasing concentrations of ghrelin (AG, 10−11 M–10−7 M) on osteoprotegerin (OPG) expression measured by real-time PCR at 24 h after AG treatment in primary cultures of rat osteoblast-like cells (a). Effect of pretreatment (30 min before) with the GHSR-1a antagonist D-Lys3-GHRP-6 (D, 10−7 M) on the stimulatory action of AG (10−10 M for 24 h) on OPG expression (b). Ratio of RANKL/OPG relative expression measured in untreated and AG 10−10 M treated rat osteoblasts after 24 h (c). OPG and RANKL expression were normalized to that of GAPDH and expressed as . Three replicates were performed for each experimental point and experiments were repeated three times. ; versus untreated cells. versus AG treated cells.
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