Research Article
Ghrelin Increases Beta-Catenin Level through Protein Kinase A Activation and Regulates OPG Expression in Rat Primary Osteoblasts
Figure 7
Effect of conditioned media (CM) obtained from rat osteoblast-like cells (rOB) treated without or with ghrelin (AG, 10−10 M for 24 or 48 h) on osteoclast formation, in presence of 50 ng/mL RANKL and 25 ng/mL M-CSF. Black arrows: multinucleated tartrate-resistant acid phosphatase positive (TRAP+) cells (a). The percentage of TRAP+ multinucleated cells (≥3 nuclei) formed after 7 days culture with conditioned media obtained from untreated and treated (AG, 10−10 M for 24 or 48) rOB (b). versus CM of untreated cells; versus CM of rOB treated for 24 h with AG.
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