Regulation of the Human Ghrelin Promoter Activity by Transcription Factors, NF-κB and Nkx2.2

Figure 1

Expression of ghrelin mRNA in cultured cell lines. PCR products were separated by agarose gel electrophoresis and stained with ethidium bromide. Human ghrelin primer set was used for COS-7, TT, ECC10, HEK293, and Hep-G2 cells. Mouse ghrelin primer set was used for AtT20 cell. Controls are human GAPDH for COS-7, TT, ECC10, HEK293, and Hep-G2 cells and mouse ribosomal RNA18 for AtT-20 cell.