Effects of Ghrelin on Triglyceride Accumulation and Glucose Uptake in Primary Cultured Rat Myoblasts under Palmitic Acid-Induced High Fat Conditions

Figure 2

Effects of acylated ghrelin on palmitic acid- (PA-) induced triglyceride (TG) accumulation in primary cultured rat myoblasts. Rat myoblasts in the high fat group were cultured with 0.3 mM PA (containing 0.5% BSA) for 12 h (a). In the three intervention groups, acylated ghrelin at 10⁻¹¹ M (b), 10⁻⁹ M (c), or 10⁻⁷ M (d) was added under high fat conditions for 12 h. TG accumulation (blue arrows) was determined by Oil-Red-O staining (scale bar: 100 μm).

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