Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus

<div>Analysis by SDS-PAGE of AGE-modified-Tg (in the presence of glucose, without pH adjustment at 7.4). Native Tg and AGE-Tg produced after incubation of Tg with three different concentrations of D-glucose-6-phosphate (no pH adjustment at 7.4) were analyzed by SDS-PAGE, samples of 10 <i>μ</i>g, in a 10% separating gel under denaturing, nonreducing conditions and then stained with Coomassie blue. Lane 1: molecular weight markers (94, 67, 43, and 30 KDa), lane 2: native Tg (330 KDa), lane 3: AGE-Tg produced with 0.03 M D-glucose-6-phosphate, lane 4: AGE-Tg produced with 0.05 M D-glucose-6-phosphate, and lane 5: AGE-Tg produced with 0.11 M D-glucose-6-phosphate.</div>

International Journal of Endocrinology

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Figure 1

Figure 1: Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus 

Figure 1 | Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus 