Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity

Table 1

Primers used for quantitative real-time RT-PCR (qRT-PCR) and bisulfite sequencing.

Primers for CRH mRNA qRT-PCR
Forward	5′-TCCCATCTCCCTGGATCTCAC-3′	GeneBank number NM_00756
Reverse	5′-GTGAGCTTGCTGTGCTAACTGCT-3′
Primers for CRH promoter bisulfite sequencing
1st PCR	Forward	5′-TTTGGGAAATTTTATTTAAGAATTTTT-3′
	Reverse	5′-CTAAATTTCTCCACTCCAAAACCTA-3′
2nd (nested) PCR	Forward	5′-GTTAATGGATAAGTTATAAGAAGTTTTT-3′
	Reverse	5′-TCCACTCCAAAACCTAAAATAAAAT-3′