Role of Nox2 and p22phox in Persistent Postoperative Hypertension in Aldosterone-Producing Adenoma Patients after Adrenalectomy

Figure 1

Activity and expression of Nox in different adrenal tissues. (a) Nox activity was detected in normal adrenocortical tissue, APA, and NFA by lucigenin chemiluminescence. (b) Expression of Nox1–Nox5, Duox1, Duox2, and p22phox mRNA in normal adrenocortical tissue and APA by RT-PCR. Signals for Nox1, Nox2, Nox4, Duox1, and p22phox were detected in normal adrenal tissue and APA. (c–h) Relative expression of Nox1, Nox2, Nox4, Duox1, p22phox, and CYP11B2 in normal adrenocortical tissue, APA, and NFA measured by Q-PCR. Nox2, p22phox, and CYP11B2 were primarily expressed in APA. (i-j) Correlation between CYP11B2 mRNA with Nox2, CYP11B2, and p22phox mRNA in APA. A positive correlation between Nox2 and CYP11B2 mRNA was found. (k–n) Western blot analysis of Nox2 and p22phox in normal adrenocortical tissue, APA, and NFA. Nox2 and p22phox were enhanced in APA compared with normal adrenocortical tissue and NFA. Results of densitometric analysis of Nox2 and p22phox proteins normalized to GAPDH were shown.

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