Role of Nox2 and p22phox in Persistent Postoperative Hypertension in Aldosterone-Producing Adenoma Patients after Adrenalectomy

Figure 2

Pathological and immunohistochemical findings of Nox2 in normal adrenocortical tissue, APA, and NFA. (a, d, g, j, and m) HES staining of normal adrenocortical tissue (a), APA (d and g), and NFA (j and m). (b and c) Nox2 and Dab2 were expressed in normal adrenocortical ZG cells. However, Nox2 was also restricted to the ZR. (d–o) Nox2 and Dab2 expression were detectable and visible beneath the capsule (h, i, k, and l) and along the vasculature (e, f, n, and o) in APA and NFA as indicated by HES. Moreover, higher magnification revealed predominant cytoplasmic localization of Nox2 and Dab2. (p) Graphical illustration of statistical distributions of Nox2 in normal adrenocortical tissue, APA, and NFA. The Nox2 IRS (immunoreactive score) was higher in APA compared to those in normal adrenal tissue and NFA. Bar, 200 μm on low magnification (100x) and 50 μm on high magnification (400x).