Research Article
Tetrahydro-iso-alpha Acids Antagonize Estrogen Receptor Alpha Activity in MCF-7 Breast Cancer Cells
Figure 6
Effect of THIAA on E2-stimulated MCF-7 cell clonogenicity in steroid-free culture medium. Cells were seeded in Petri dishes at low concentration. Cells were grown during 72 h in the absence (control) or presence of E2 at 10−9 M with or without antiestrogens at 10−6 M or THIAA at 0.01%. Number of colonies (a) and number of cells per colony (b) were counted. For the later, data were subdivided into 4 classes (1-2, 3–6, 7–11, and more than 11 cells) according to a quartile calculation.
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