Research Article
Tetrahydro-iso-alpha Acids Antagonize Estrogen Receptor Alpha Activity in MCF-7 Breast Cancer Cells
Figure 7
Effect of THIAA on ERα-dependent transcription by reporter gene assay in steroid-free culture medium. MVLN cells were incubated for 24 h in the absence (control) or presence of E2 at 10−9 M with or without THIAA at 0.001%. Luciferase activity was assayed in cellular extracts by luminometry and emitted light signals were expressed in arbitrary units (relative luciferase units (RLU)) per mg protein. Data refer to the mean value ± SD of an experiment performed in triplicate and are representative of three independent experiments.