Rapid Action of Aldosterone on Protein Levels of Sodium-Hydrogen Exchangers and Protein Kinase C Beta Isoforms in Rat Kidney

Table 2

Median staining intensity (score) of renal PKCβI, PKCβII, NHE1, and NHE3 protein localization.

	Median staining intensity (score)
	PKCβI		PKCβII		NHE1		NHE3
	Sham	Aldo	Sham	Aldo	Sham	Aldo	Sham	Aldo
Cortex
Glomerulus	2	3	3	3	3	3	1	2
PCT	0	0	1	1	1	2	1	1
DCT	0	0	0	0	1	2	1	1
CCD	0	0	0	0	3	4	1	2
Pcap	2	3	3	3	2	2	1	1
Outer medulla
TALH	1	1	2	1	1	1	2	1
MCD	1	1	3	1	3	4	2	1
VR	3	4	3	3	1	4	2	4
tLH	3	4	2	3	1	4	2	4
Inner medulla
MCD	1	1	3	1	3	4	1	0
VR	1	2	1	1	1	2	1	4
tLH	1	2	2	1	1	2	1	2

Staining intensity: 0 = negative, no reactivity; 1 = trace, faint or pale brown staining with less membrane reactivity; 2 = weak, light brown staining with incomplete membrane reactivity; 3 = moderate, shaded of brown staining of intermediate darkness with usually almost complete membrane reactivity; 4 = strong, dark brown to black staining with usually complete membrane pattern, producing a thick outline of the cell [11–13]. PCT: proximal convoluted tubule; DCT: distal convoluted tubule; CCD: cortical collecting duct; Pcap: peritubular capillary; TALH: thick ascending limb of the loop of Henle; MCD: medullary collecting duct; VR: vasa recta; tLH: thin limb of the loop of Henle ().