Crosstalk between Aryl Hydrocarbon Receptor and Glucocorticoid Receptor in Human Retinal Pigment Epithelial Cells

Figure 1

Crosstalk of AHR- and GR-mediated expression in ARPE-19. (a) ARPE-19 cells were treated with TCDD (10 nM), Dex (100 nM), or TCDD (10 nM) + Dex (100 nM). For the combination treatment, ARPE-19 cells were pretreated with TCDD for 2 h before Dex was added. 6 h after TCDD treatment, total RNA was isolated. The levels of CYP1A1, CYP1B1, and AHRR mRNA were normalized to the 18S rRNA level. (b) ARPE-19 cells were pretreated with Dex (10 nM) for 2 h before TCDD (30 nM) was added. 4 h after Dex treatment, total RNA was isolated. The levels of ANGPTL4, FKBP5, and GILZ mRNA were normalized to the 18S rRNA level. The results are presented as the mean ± SD of three independent experiments (). , , and .

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