Crosstalk between Aryl Hydrocarbon Receptor and Glucocorticoid Receptor in Human Retinal Pigment Epithelial Cells

Figure 2

Dex inhibits the nuclear translocation of AHR in ARPE-19. (a) ARPE-19 cells were treated for 30 min to Dex (100 nM), TCDD (30 nM), or Dex (100 nM) + TCDD (30 nM). Western blot analysis of GR and AHR in cytoplasmic and nuclear fraction was performed. Histone H3 is used as a control for a nuclear fraction, and α-tubulin is used as a control for cytoplasmic fraction. (b) The relative protein levels of GR and AHR were quantified by scanning densitometry and normalized to α-tubulin (cytoplasmic fraction) and histone H3 (nuclear fraction), respectively. The images shown are representatives of three independent experiments that showed consistent results, and the relative protein values are expressed as mean ± SD for three independent experiments. and

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