Crosstalk between Aryl Hydrocarbon Receptor and Glucocorticoid Receptor in Human Retinal Pigment Epithelial Cells

Figure 3

Decrease of AHR recruitment to its target genes by Dex. (a) AHR recruitment to AHR target genes. ARPE-19 cells were treated with TCDD (30 nM), Dex (100 nM), or TCDD (30 nM) + Dex (100 nM) for 30 min. Cells were cross-linked using formaldehyde and subjected to ChIP using antibodies against AHR. Immunoprecipitated DNA was quantified by qPCR using primers for CYP1A1 (−12 kb), CYP1B1, and AHRR. (b) Chromatin accessibility at the AHR target genes was assessed by FAIRE-qPCR analysis by using chromatin samples prepared from ARPE-19 cells treated with TCDD (30 nM), Dex (100 nM), or TCDD (30 nM) + Dex (100 nM) for 30 min. Data are normalized against non-cross-linked genomic DNA for each primer pair. The results are presented as the mean ± SD of three independent experiments (). , , and .

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