Crosstalk between Aryl Hydrocarbon Receptor and Glucocorticoid Receptor in Human Retinal Pigment Epithelial Cells

Figure 4

AHR increases GR recruitment on GBS of GR target genes. (a) and (b) ARPE-19 cells were treated with Dex (2.5 nM), TCDD (30 nM), or Dex (2.5 nM) + TCDD (30 nM). For the combination treatment, ARPE-19 cells were treated with Dex for 5 min before TCDD treatment. After TCDD treatment for 10 min, cells were cross-linked using formaldehyde and subjected to ChIP using antibodies against GR and AHR. Immunoprecipitated DNA was quantified by qPCR using primers for ANGPTL4 (GBS) and FKBP5 (GBS). The results are presented as the mean ± SD of three independent experiments (). . GBS: GR-binding sites.

(a)

(b)