Prolonged Survival of Subcutaneous Allogeneic Islet Graft by Donor Chimerism without Immunosuppressive Treatment

Figure 3

Immunohistochemical examination of the retrieved islet grafts. The graft-bearing kidneys and the subcutaneous grafts from the mice in groups A, B, C, and D were retrieved at 20 to 30 weeks posttransplantation. The immunohistochemical examinations were performed using anti-insulin antibodies. The secondary antibody was prepared from rabbits and conjugated with horseradish peroxidase, and diaminobenzidine was used as the chromogenic substance. The dark-brownish precipitates indicate insulin-containing cells. (a), (b), (c), and (d) show the graft histology of mice in groups A, B, C, and D, respectively. Photos are 40 × 10 magnification. Scale bars indicate 0.1 mm.

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