Prolonged Survival of Subcutaneous Allogeneic Islet Graft by Donor Chimerism without Immunosuppressive Treatment

Table 3

T cell subsets were identified by flow cytometry analysis.

T subset (%)	Group A; Renal site; ()		Group B; Subcutaneous site; ()		Group D; Subcutaneous site; ()
Time	2 weeks	8 weeks	2 weeks	8 weeks	2 weeks	8 weeks
CD4-TN	31.1 ± 5.4	66.5 ± 9.2	38.4 ± 11.3	59.6 ± 15.9	60.8 ± 7.9	31.2 ± 9.5
CD4-TEM	37.7 ± 5.5	14.6 ± 8.9	35.8 ± 8.6^a	24.6 ± 13.8	12.0 ± 3.8^a	15.3 ± 1.9
CD4-TCM	5.5 ± 1.0	9.9 ± 1.5	5.6 ± 1.0	9.1 ± 1.6	8.7 ± 1.0	9.2 ± 2.5
CD8-TN	1.4 ± 0.3	47.4 ± 9.5	3.4 ± 1.5^b	34.4 ± 15.9	15.7 ± 2.8^b	12.4 ± 4.3
CD8-TEM	90.7 ± 0.9	29.6 ± 9.4	86.0 ± 1.9^c	49.9 ± 17.2	41.9 ± 6.1^c	31.8 ± 3.8
CD8-TCM	4.3 ± 0.4	16.0 ± 1.5	7.9 ± 1.2^d	13.9 ± 2.4	21.7 ± 3.5^d	22.2 ± 3.3

Naive T cells (TN), central memory T cells (TCM), and effector memory T cells (TEM) were defined as CD44^loCD62L⁺, CD44^hiCD62L⁺, and CD44^hiCD62L⁻, respectively. ^a, ^b, ^c, and ^d comparing the indicated two means using independent t-test.