Hydrogen Sulfide Inhibits High Glucose-Induced sFlt-1 Production via Decreasing ADAM17 Expression in 3T3-L1 Adipocytes

Figure 2

Effects of high glucose on sFlt-1 production in ADAM17 knockdown 3T3-L1 adipocytes. 3T3-L1 adipocytes were transfected with NC siRNA exposed to LG DMEM, NC siRNA exposed to HG DMEM, or ADAM17 siRNA exposed to HG DMEM. (a) Representative protein bands of pro-ADAM17 and active ADAM17 in 3T3-L1 adipocytes transfected with NC siRNA and ADAM17 siRNA exposed to HG DMEM. The protein expression of ADAM17 in 3T3-L1 adipocytes was determined by Western blotting. (b) The effects of high glucose on sFlt-1 production in ADAM17 knockdown 3T3-L1 adipocytes. The production of sFlt-1 in the media was measured by ELISA. Data were presented as mean ± SEM ( cultures). , versus indicated.

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