Hydrogen Sulfide Inhibits High Glucose-Induced sFlt-1 Production via Decreasing ADAM17 Expression in 3T3-L1 Adipocytes

Figure 3

Effects of high glucose on sFlt-1 production in ADAM17-blocked or -inhibited 3T3-L1 adipocytes. (a) The effects of high glucose on sFlt-1 production in ADAM17-blocked 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with LG DMEM containing nonspecific IgG or HG DMEM containing nonspecific IgG or HG DMEM containing anti-ADAM-17 monoclonal antibody (D1(A12)). (b) The effects of high glucose on sFlt-1 production in ADAM17-inhibited 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with LG DMEM containing DMSO or HG DMEM containing DMSO or HG DMEM containing TAPI-1. The production of sFlt-1 in the media was measured by ELISA. Data were presented as mean ± SEM ( cultures). versus indicated.

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