Hydrogen Sulfide Inhibits High Glucose-Induced sFlt-1 Production via Decreasing ADAM17 Expression in 3T3-L1 Adipocytes

Figure 5

Effects of decreasing CSE and 3-MST expression on the expression of ADAM17 and production of sFlt-1 in 3T3-L1 adipocytes. 3T3-L1 adipocytes were transfected with negative control (NC) siRNA, CSE siRNA, or 3-MST siRNA for 24 h. (a and b) Effect of decreasing CSE (a) and 3-MST (b) expression on the protein expression of pro-ADAM17 and active ADAM17 in 3T3-L1 adipocytes. The protein expression of pro-ADAM17 and active ADAM17 in 3T3-L1 adipocytes was determined by Western blotting as described in Materials and Methods. Representative protein bands were presented on the top of the corresponding histogram. (c and d) Effect of decreasing CSE (c) and 3-MST (d) expression on the production of sFlt-1. The production of sFlt-1 in the media was measured by ELISA. Data were presented as mean ± SEM ( cultures). , versus indicated.

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