Metformin Regulating miR-34a Pathway to Inhibit Egr1 in Rat Mesangial Cells Cultured with High Glucose

Figure 7

Egr1 gene silencing suppresses the Egr1 protein expression (by western blotting) stimulated by high glucose (a). Egr1 gene silencing suppresses the upregulated expression of fibrosis factors (FN and CTGF) (b) (by western blotting) and inflammatory factors (MCP-1 and CXCL5) (c) (by ELISA) stimulated by high glucose in RMCs. RMCs were transfected with nonspecific siRNA (N-siRNA) or three different siRNA targeting Egr1 gene (E1-siRNA, E2-siRNA, and E3-siRNA) for 6 hours and then cultured with high glucose media (30 mmol/L glucose) or normal glucose (NG, 5.5 mmol/L glucose) for 24 hours. , versus RMCs cultured with high glucose media. ^# versus RMCs cultured with normal glucose media. All results represent means ± SD obtained from three independent experiments in triplicate and normalized to GAPDH. Egr1: early growth response factor 1; FN: fibronectin; CTGF: connective tissue growth factor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; MCP-1: monocyte chemoattractant protein 1; CXCL5: chemokine C-X-C motif ligand 5.

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