Phylogenetic and Guanine-Cytosine Content Analysis of Symbiobacterium thermophilum Genes

Figure 1

Pie chart of the categories of the 3338 Symbiobacterium protein-coding genes. A BLAST search was conducted for all proteins from 147 eukaryotes, 1047 bacteria, and 84 archaea in the KEGG database (http://www.kegg.jp) considering the parameter values given on the GenomeNet website (http://www.genome.jp). The query amino acid sequence was each protein of Symbiobacterium thermophilum. The top hit (best match) for each Symbiobacterium protein was recorded. However, if the top hit was absent or if the E-value of the top hit exceeded 0.1, the Symbiobacterium protein was considered to have no similar protein (category, “No hit”). We categorized the 3338 Symbiobacterium proteins into the following 17 categories: “Actinobacteria,” “Aquificae,” “Archaea/Eukaryota,” “Bacilli,” “Bacteroidetes/Chlorobi,” “Chloroflexi,” “Clostridia,” “Cyanobacteria,” “Deinococcus-Thermus,” “Dictyoglomi,” “Fibrobacteres/Acidobacteria,” “No hit,” “Proteobacteria,” “Symbiobacterium,” “Thermobaculum,” “Thermotogae,” and “Other bacteria.” If the top hit was another protein(s) of Symbiobacterium, then the query protein was considered to belong to the category “Symbiobacterium.”