- About this Journal ·
- Abstracting and Indexing ·
- Advance Access ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
International Journal of Evolutionary Biology
Volume 2012 (2012), Article ID 740605, 9 pages
A Phenotypic Point of View of the Adaptive Radiation of Crested Newts (Triturus cristatus Superspecies, Caudata, Amphibia)
1Institute of Zoology, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia
2Department of Evolutionary Biology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11000 Belgrade, Serbia
Received 12 July 2011; Revised 6 October 2011; Accepted 11 October 2011
Academic Editor: Kyoichi Sawamura
Copyright © 2012 Ana Ivanović et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The divergence in phenotype and habitat preference within the crested newt Triturus cristatus superspecies, examined across different ontogenetic stages, provides an excellent setting to explore the pattern of adaptive radiation. The crested newts form a well-supported monophyletic clade for which at least the full mitochondrial DNA phylogeny is resolved. Here we summarise studies that explored the variation in morphological (larval and adult body form, limb skeleton, and skull shape) and other phenotypic traits (early life history, developmental sequences, larval growth rate, and sexual dimorphism) to infer the magnitude and direction of evolutionary changes in crested newts. The phenotypic traits show a high level of concordance in the pattern of variation; there is a cline-like variation, from T. dobrogicus, via T. cristatus, T. carnifex, and T. macedonicus to the T. karelinii group. This pattern matches the cline of ecological preferences; T. dobrogicus is relatively aquatic, followed by T. cristatus. T. macedonicus, T. carnifex, and the T. karelinii group are relatively terrestrial. The observed pattern indicates that phenotypic diversification in crested newts emerged due to an evolutionary switch in ecological preferences. Furthermore, the pattern indicates that heterochronic changes, or changes in the timing and rate of development, underlie the observed phenotypic evolutionary diversification.
Exploring patterns of phenotypic variation during ontogeny and phylogeny is fundamental to gaining insights into the processes of evolutionary diversification, including the mechanisms of speciation. The connection between development, evolutionary history, ecology, and morphology has intrigued evolutionary biologists for over the 150 years since Darwin first published his ideas about natural selection . This is largely due to the idea that phenotypic evolution might be explained by changing or truncating the ancestral ontogeny, for which the characteristics can be inferred through phylogenetic analyses [2–4].
Within a monophyletic group of closely related species, it is expected that shared evolutionary history is reflected by phenotypic similarity, due to a shared developmental basis inherited from a common ancestor. Adaptive radiation and morphological divergence are usually attributed to differential selection acting upon geographical populations. In other words, “ecological opportunity” could lead to adaptive radiation [5, 6]. When phenotypic divergence is paralleled in multiple complex phenotypic traits with separate developmental pathways, this is indicative of adaptation to differential environmental selection pressures [6–9]. This line of reasoning is especially strong in situations where phenotypic variation correlates with different ecological demands.
The crested newts (Triturus cristatus superspecies) are an example of an adaptive radiation. Crested newts have been used as model organisms in various studies in evolutionary biology, including the processes and outcomes of speciation [10–14]. The phenotypic and ecological divergence in crested newts, examined across different ontogenetic stages, provides an excellent model to explore the tradeoff between shared evolutionary history and divergent functional requirements within an adaptive radiation.
Here we summarise data from previous studies on crested newts. The resulting framework can be used to evaluate how developmental and functional processes have impacted phenotypic evolution.
2. About Crested Newts
Crested newts belong to the group of the Modern Eurasian newts . Crested newts have a biphasic life cycle with aquatic larvae that metamorphose into terrestrial juveniles and as adults return to the water annually to breed [34, 35]. Crested newts have low mobility, a small dispersal range, and strong philopatric behaviour, which promote genetic isolation. The crested newts form a well-supported monophyletic clade of closely related species for which the molecular phylogeny has been largely resolved [11, 14, 33, 36]. According to current taxonomy, this group consists of six species: T. cristatus, T. dobrogicus, the closely related species T. carnifex and T. macedonicus , and two species that belong to the so-called T. karelinii group, T. karelinii and T. arntzeni. Mitochondrial DNA studies showing three distinct clades within the T. karelinii group [14, 15] illustrate that the taxonomy of the T. karelinii clade is as yet unsettled. In an attempt to simplify, we will refer to these newts as the T. karelinii group.
The range of the crested newts spans most of Europe and adjacent Asia (Figure 1). The nominotypical species (T. cristatus) is the most widely distributed over much of Europe. T. dobrogicus is confined to the Pannonian area and the Danube Delta and Dobrugea Plain. The other species have a more southern distribution, restricted to the Apennine Peninsula and the northeast Balkan Peninsula (T. carnifex), the western Balkan Peninsula (T. macedonicus) and the eastern Balkan Peninsula, Asia Minor, Crimea, Caucasus, and southern shore of the Caspian Sea (T. karelinii group). The species have a parapatric distribution and a potential to interbreed along the contact zones, especially in the Balkan region [37, 38].
The crested newt species differ in their ecological demands [18, 19]. The most aquatic is T. dobrogicus, followed by T. cristatus. Generally, T. macedonicus, T. carnifex and the T. karelinii group are the more terrestrial species. T. dobrogicus inhabits permanent and/or long-lasting, large, stagnant water bodies. T. cristatus occupies mostly long-lasting medium-sized water bodies. The other crested newt species are associated with relatively small lentic ponds with variable hydroperiods [18, 37]. The duration of the aquatic phase is directly related to their habitat and varies from a short, three months in the T. karelinii group up to six months in T. dobrogicus (Figure 2). It is worth to note that the evolution of habitat preferences of crested newts still needs to be addressed properly.
The most notable characteristic of the crested newts’ origin is that their evolutionary splitting occurred within a short-time span, which indicates that there was a burst of speciation rather than a prolonged process of speciation [11, 14, 36]. This is typical for adaptive radiations. Ecologically based, spatially heterogeneous selection, coupled with limited migration, can result in rapid phenotypic diversification [39, 40]. Such a scenario presumes the existence of divergent ecological conditions, as well as a low magnitude of phenotypic and genetic correlations. A high level of phenotypic divergence could be achieved even under substantial hybridisation and gene flow .
3. Interspecific Variation in Phenotypic Traits
3.1. Adult Body Form
The term “body form” refers to the robust morphological features of an organism’s external morphology and encompasses both size- and shape-related characteristics. Body form can differ between species, but also between groups of species. In tailed amphibians, adaptation to an aquatic life is usually related to body elongation and limb reduction, which may increase swimming performance. A compact body and robust limbs are linked to a terrestrial life and a lack of passive buoyancy.
Crested newts show a range body forms from a slender body and short limbs in T. dobrogicus, via T. cristatus, and T. macedonicus and T. carnifex to a short body and long limbs in the T. karelinii group [37, 42]. The ancestral phenotype, a large body with a short trunk and a wide head, characterises the T. karelinii group. The species T. carnifex and T. macedonicus have a large body and wide head accompanied by mild body elongation. The most derived phenotype includes body size reduction and more pronounced body elongation in T. cristatus and, especially, in T. dobrogicus (Figure 2). Body elongation in these newts is reflected in the modal number of rib-bearing vertebrae: 13 in T. karelinii group, 14 in T. macedonicus and T. carnifex, 15 for T. cristatus, and 16 or 17 in the most elongated T. dobrogicus [14, 37, 43, 44].
3.2. Ontogeny of Body Shape
The ontogenetic niche shift and transition between the aquatic and terrestrial habitats is coupled with metamorphosis and an overall change in the relationship between the individual and its environment. Therefore, two different sets of adaptations and constraints during growth could shape the ontogenetic trajectories of crested newts and affect their phenotypic diversification. The analysis of ontogenetic shape changes and changes in developmental rate  gives insight into the processes of the evolutionary diversification of the crested newts. The four analysed species of crested newts (T. dobrogicus, T. cristatus, T. macedonicus, and T. arntzeni (T. karelinii group), differ in size and shape as larvae, at least when the larval body form is fully developed (i.e., midlarval stage). The ontogenetic trajectories of larval shape diverge in both the direction and the rate of shape changes along species-specific trajectories . The species significantly differ in their developmental rate of larval shape, except for T. cristatus and T. dobrogicus, which are similar. T. dobrogicus clearly differed from the other species in having a higher and wider caudal fin, while T. arntzeni (T. karelinii group) has the most elongated larvae with the lowest tail fin (Figure 3). Based on the assumption that the shape of caudal fins is of high adaptive significance for larvae, it is tempting to hypothesise that the two larval shape types represent aquatic ecological adaptations of the two species groups of crested newt.
Contrary to clear discrimination between species in larval body shape, at the juvenile stage just after metamorphosis, the species converge on a similar body shape . After that stage, crested newt species apparently diverge toward the adult body shape. The differences in body shape that we found may indicate that the body forms of larvae and adult individuals are subject to selection in both the aquatic and terrestrial environments, resulting in the same pattern of interspecific differences, despite the possibility of two distinct sets of constraints .
3.3. Limb Size, Ossification Level, and the Pattern of Morphological Integration
A morphometric analysis of the limb skeleton of four crested newt species (T. dobrogicus, T. cristatus, T. carnifex, and T. arntzeni (T. karelinii group)) showed that although they differ in the size of skeletal elements (stylopodium, zeugopodium, and third metapodial element), they all shared common allometric slopes . A similar relationship between limb skeleton size and body size could indicate a conservative direction of ontogeny . However, a lateral shift in the species-specific allometries of T. dobrogicus and T. cristatus indicates evolutionary changes in the allometric trajectories. The lateral shift could indicate that heterochronic changes underlie the observed morphological variation . Moreover, the T. dobrogicus manus has a significantly lower ossification level and concomitant loose “bone packaging” compared with the other species (Figure 4), which could be a result of the heterochronic changes [46, 47].
Limbs, as serially homologous structures, share a strong developmental basis. The shared genetic factors (e.g., Hox patterning genes) are intrinsic to the covariation among the homologous structures within limbs (e.g., radius and tibia or humerus and femur) and the overall morphological integration [48, 49]. Epigenetic factors, such as function, also could have an impact on limb integration. In empirical studies, the expression of functional and developmental interdependencies in the patterns of integration could be estimated. If covariation between the homologous parts of the fore- and hindlimbs is stronger than the covariation of skeletal elements within the limbs, then developmental constraints prevail over functional determinants [49–51]. Two opposing correlation patterns were observed  in the more terrestrial species, the homologous limb elements were less correlated, and the within-limb elements were more correlated, whereas, in the aquatic species, the reverse pattern occurred (Figure 5). All of these results indicate that function appears to be the covariance-generating factor that has shaped the patterns of morphological integration of crested newt limbs.
3.4. Skull Shape and Ontogenetic Skull Shape Changes
Crested newts differ in skull shape . The visualisation of the phylogeny superimposed in the morphospace, and the positions of the internal nodes in the phylomorphospace (Figure 6) indicate that most of the shape changes occurred along species-specific branches. T. dobrogicus markedly diverged in skull shape as reflected by a more slender and elongated skull (Figure 6). The advantages of such morphology might be better locomotion in aquatic habitats due to a more streamline body shape but with the possible disadvantage of reduced abilities of suction feeding. The similarities in skull shape of T. macedonicus and T. arntzeni (T. karelinii group) probably reflect a symplesiomorphy.
The analysis of the ontogenetic trajectories of skull shape changes between juveniles just after metamorphosis and adults  indicate that T. dobrogicus has the highest rate of cranial shape change during postmetamorphic growth, as well as a distinctive ontogenetic allometric trajectory compared with the other three analysed species (T cristatus, T. carnifex, and T. arntzeni (T. karelinii group)). To visualise the direction and amount of shape changes during crested newt skull shape ontogeny (Figure 7), we performed an additional analysis using a larger sample of juveniles than available for the study of ontogenetic shape changes . The slope and the amount of ontogenetic shape change of T. dobrogicus clearly diverged from the other species. It is interesting to note that the changes in skull shape that clearly separate T. dobrogicus and T. cristatus from T. macedonicus and the T. karelinii group are shared in both analysed stages: the juvenile stage just after metamorphosis and the adult stage (Figure 7).
3.5. Early Ontogeny
Comparative study of crested newt development and early life history traits, such as egg characteristics, developmental rate, survival rate, and duration of the embryonic period [52, 53] produced data valuable for understanding the forces shaping adaptation and evolutionary diversification. In vertebrates, vitellus size appears to be one of the key life-history traits reflecting maternal input and could affect the development rate and the size and stage of larvae at hatching . The vitellus size and thickness of the mucoid capsule that protects from injury, fungal infestation, and ultraviolet-B radiation were investigated in four crested newt species (T. macedonicus, T. cristatus, T. dobrogicus, and T. arntzeni (T. karelinii group)) . Larger crested newt females tend to produce eggs with larger vitelluses. Although the studied species shared a common allometric slope of the egg size versus body size relationship, the species differed in the egg size, which appeared to be a species-specific life-history trait with a cline-like distribution; T. dobrogicus has the smallest eggs, the members of the T. karelinii group and T. macedonicus have the largest eggs, and T. cristatus has intermediate-sized eggs.
The crested newts are similar with respect to basic developmental traits (no differences in developmental sequences and survival rates). However, there is a significant variation in the developmental rate. Generally, the developmental rate highly depends on environmental factors, especially temperature. Under experimental conditions , T. dobrogicus appears to be the outlier species, particularly in comparison to T. arntzeni (T. karelinii group) and T. macedonicus, which have the longest developmental period . Also, there are differences in the pattern of correlation amongst life-history and developmental traits. The comparisons of phenotypic correlation matrices based on eleven life history and developmental traits  revealed that T. dobrogicus have a correlation pattern similar to T. cristatus and T. macedonicus. T. arntzeni (T. karelinii group) has a similar correlation pattern to T. macedonicus, but there are no similarities in the matrix correlation pattern compared to the other two species.
4. Adaptive Radiation Pattern
Adaptive radiation refers to the evolution of ecological and phenotypic diversity within multiple lineages . There are four criteria to detect an adaptive radiation ; common ancestry, rapid radiation, environmental correlation, and trait utility. In crested newts, common ancestry and rapid radiation can be readily inferred from phylogenetic analysis. Also, there is a perfect match between the patterns of interspecific differentiation in phenotypic traits and ecological preferences. However, no clear relationship between evolution of body form and locomotor function in newts was found . The onset of adaptive radiation often requires, in addition to the existence of a new habitat, the possession of a key innovation that allows rapid adaptation in novel ecological settings .
The observed pattern of differences in the crested newts’ phenotypic characteristics shows that T. dobrogicus is in any respect the most derived species. T. dobrogicus has (1) the most elongated body and the largest number of rib-bearing vertebrae [14, 42]; (2) a significantly different size, ossification level, and pattern of morphological integration of limbs [13, 30]; (3) a marked difference in skull shape , including the direction and rate of ontogenetic shape changes ; (4) the smallest vitellus ; (5) peculiarities in life history traits ; (6) a distinct pattern of sexual dimorphism of morphometric traits (the sexual dimorphism in body size is absent in T. dobrogicus, while in other species females are the larger sex ). However, from this long list of diverged phenotypic traits that characterise T. dobrogicus, a key innovation cannot yet be clearly recognised.
5. Possible Mechanism of the Crested Newts’ Evolutionary Diversification in Phenotypic Traits
External restrictions imposed by ecology had a strong influence on the crested newts’ phenotypic divergences, including development. In our view, the evolutionary diversification of phenotypic traits in crested newts was driven by ecological speciation. (An additional separation between crested newts might occur through parapatric speciation, in which populations diverge with some gene flow .)
The pattern of divergence in developmental rate and correlation pattern between several early life-history and developmental traits [52, 53] indicates that crested newt evolution seemed to be accompanied by a significant ecological diversification and by labile development patterning, including differences in developmental timing. Heterochrony, differences in the sequence of developmental events, the timing and the rates of development, are often invoked as causes that underlie observed phenotypic evolutionary changes . Crested newts are prone to heterochronic changes . The data collected so far suggest that heterochronic changes in early ontogeny can lead to the lateral transposition of the T. dobrogicus ontogenetic trajectories, as previously suggested for the cranial shape  and allometric limb skeleton trajectories .
6. The Ecological Shift Drives the Evolution of Phenotypic Traits in the Crested Newts
Natural selection related to shifts in ecology (e.g., invasion of new habitats) can lead to extremely rapid divergence . The new, colonising populations are particularly likely to diverge, especially because they are usually small and likely to be genetically altered. Recently, it has been proposed that new species generally emerge from single events (e.g., changes in environments), and that ecological adaptation promotes reproductive isolation and speciation [58, 64, 65]. Also, a growing body of research demonstrates a link between rapid ecological divergence and speciation .
We advocate the hypothesis that the phenotypic diversification in crested newts emerged due to an evolutionary switch in ecological preferences . The phenotypic characteristics of crested newts could have evolved over a short-time span during which the main crested newts phylogenetic lineages diverged in the central Balkans [11, 43]. The main ecological shift of T. dobrogicus (and less apparently of T. cristatus) could happen due to the availability of aquatic habitats on the Central Balkans [67, 68]. The subsequent phenotypic evolution of T. dobrogicus could be amplified through ecological selection, when the species occupied extensive lowland floodplains along the present-day Danube River and its tributaries which were covered with swamps and marshes. This event dated back to Pliocene when the Pannonian Sea dried out, more than three million years after T. dobrogicus had separated from other crested newts’ species . Similar changes might have occurred when T. cristatus spread across the European plains after Pleistocene glaciations. If so, adaptive phenotypic radiation in crested newts appears as an extension of the initial process of speciation.
For both T. dobrogicus and T. cristatus, the acquiring of new habitats by phenotypic diversification was not accompanied by additional lineage differentiation. Most likely, the high connectivity of large lowland water bodies, distributed in areas affected by glaciations, prevented the long-term partitioning of populations and thus inhibited speciation [69, 70]. In contrast, within the T. karelinii group, subdivision across a heterogeneous landscape, especially those in glacial refuges, promotes geographical isolation and thus speciation [15, 69].
The morphologically and ecologically diversified species of crested newts exhibit a cline-like variation pattern in phenotypic traits, with T. dobrogicus and the T. karelinii group on the opposite poles and T. cristatus as an intermediate species, while T. carnifex and T. macedonicus are close to the T. karelinii group. This pattern matches the cline of the species’ ecological preferences indicating that phenotypic diversification in crested newts emerged most likely due to an evolutionary switch in ecological preferences. The patterns of variation also indicate that heterochronic changes underlie the observed phenotypic evolutionary changes.
The authors thank Ben Wielstra and an anonymous reviewer for constructive comments and discussion. This work was supported by the Serbian Ministry of Education and Science (Grant no. 173043).
- C. Darwin, On the Origin of Species by Means of Natural Selection, or the Preservation of Favoured Races in the Struggle for Life, John Murray, London, UK, 1859.
- S. J. Gould, Ontogeny and Phylogeny, Belknap Press, Cambridge, Mass, USA, 1977.
- C. P. Klingenberg, “Heterochrony and allometry: the analysis of evolutionary change in ontogeny,” Biological Reviews of the Cambridge Philosophical Society, vol. 73, no. 1, pp. 79–123, 1998.
- M. L. Zelditch and R. A. Moscarella, “Form, function, and life history: spatial and temporal dynamics of integration,” in Phenotypic Integration: Studying the Ecology and Evolution of Complex Phenotypes, M. Pigliucci and K. Preston, Eds., pp. 274–297, Oxford University Press, Oxford, UK, 2004.
- J. B. Losos, “Adaptive radiation, ecological opportunity, and evolutionary determinism,” The American Naturalist, vol. 175, no. 6, pp. 623–639, 2010.
- D. C. C. Andrew, “The ecological causes of evolution,” Trends in Ecology and Evolution, vol. 26, no. 10, pp. 514–522, 2011.
- T. F. Hansen and E. P. Martins, “Translating between microevolutionary process and macroevolutionary patterns: the correlation structure of interspecific data,” Evolution, vol. 50, no. 4, pp. 1404–1417, 1996.
- C. P. Klingenberg, “Integration, modules and development: molecules to morphology to evolution,” in Phenotypic Integration: Studying the Ecology and Evolution of Complex Phenotypes, M. Pigliucci and K. Preston, Eds., pp. 213–230, Oxford University Press, Oxford, UK, 2004.
- L. J. Revell, L. J. Harmon, and D. C. Collar, “Phylogenetic signal, evolutionary process, and rate,” Systematic Biology, vol. 57, no. 4, pp. 591–601, 2008.
- L. Gvoždik and R. Van Damme, “Triturus newts defy the running-swimming dilemma,” Evolution, vol. 60, no. 10, pp. 2110–2121, 2006.
- J. W. Arntzen, G. E. Themudo, and B. Wielstra, “The phylogeny of crested newts (Triturus cristatus superspecies): nuclear and mitochondrial genetic characters suggest a hard polytomy, in line with the paleogeography of the centre of origin,” Contributions to Zoology, vol. 76, no. 4, pp. 261–278, 2007.
- A. Ivanović, T. Vukov, N. Tomašević, G. Džukić, and M. L. Kalezić, “Ontogeny of skull size and shape changes within a framework of biphasic lifestyle: case study of European newts (genus Triturus spp.),” Zoomorphology, vol. 126, no. 3, pp. 173–183, 2007.
- N. T. Kolarov, A. Ivanović, and M. L. Kalezić, “Morphological integration and ontogenetic niche shift: a study of crested newt limbs,” Journal of Experimental Zoology Part B, vol. 314, no. 4, pp. 296–305, 2011.
- B. Wielstra and J. W. Arntzen, “Unraveling the rapid radiation of crested newts (Triturus cristatus superspecies) using complete mitogenomic sequences,” BMC Evolutionary Biology, vol. 11, Article ID 162, 2011.
- B. Wielstra, G. E. Themudo, O. Güçlü, K. Olgun, N. A. Poyarkov, and J. W. Arntzen, “Cryptic crested newt diversity at the Eurasian transition: the mitochondrial DNA phylogeography of near eastern Triturus newts,” Molecular Phylogenetics and Evolution, vol. 56, no. 3, pp. 888–896, 2010.
- G. Džukić, Tailed Amphibians (Caudata) of Serbia. A faunistic and zoogeographical study, regarding protection and conservation, Ph.D. thesis, Univeristy of Belgrade, Belgrade, Serbia, 1993.
- M. L. Kalezić, G. Džukić, G. Mesaroš, and J. Crnobrnja-Isailović, “The crested newt (Triturus cristatus superspecies) in ex-Yugoslavia: morphological structuring and distribution patterns,” The University Thought (Priština), vol. 4, no. 1, pp. 39–46, 1997.
- J. W. Arntzen, “Triturus cristatus Superspezies—Kammolch-Artenkreis. (Triturus cristatus (Laurenti, 1768)—Nördlicher Kammolch, Triturus carnifex (Laurenti, 1768)—Italienischer Kammolch. Triturus dobrogicus(Kiritzescu, 1903)—Donau-Kammolch, Triturus karelinii (Strauch, 1870)—Südlicher Kammolch),” in Handbuch detr Reptilien und Amphibiaen Europas, Band 4/IIA: Schwanzlurche (Urodela) IIA, W. Böhme, Ed., pp. 421–514, Aula-Verlag, Wiebelsheim, Germany, 2003.
- S. N. Litvinchuk, J. M. Rosanov, and L. J. Borkin, “Correlations of geographic distribution and temperature of embryonic development with the nuclear DNA content in the Salamandridae (Urodela, Amphibia),” Genome, vol. 50, no. 4, pp. 333–342, 2007.
- A. Ivanović, M. Cvijanović, and M. L. Kalezić, “Ontogeny of body size and shape and metamorphosis: insights from the crested newts,” Journal of Zoology, vol. 283, no. 3, pp. 153–161, 2011.
- C. P. Klingenberg, “Evolution and development of shape: integrating quantitative approaches,” Nature Reviews Genetics, vol. 11, no. 9, pp. 623–635, 2010.
- F. L. Bookstein, Morphometric Tools for Landmarks Data: Geometry and Biology, vol. Cambridge University Press, Cambridge, UK, 1991.
- C. P. Klingenberg and N. A. Gidaszewski, “Testing and quantifying phylogenetic signals and homoplasy in morphometric data,” Systematic Biology, vol. 59, no. 3, pp. 245–261, 2010.
- W. P. Maddison, “Squared-change parsimony reconstructions of ancestral states for continuous-valued characters on a phylogenetic tree,” Systematic Zoology, vol. 40, no. 3, pp. 304–314, 1991.
- B. H. Mcardle and A. G. Rodrigo, “Estimating the ancestral states of a continuous-valued character using squared-change parsimony: an analytical solution,” Systematic Biology, vol. 43, no. 4, pp. 573–578, 1994.
- F. J. Rohlf, “Comparative methods for the analysis of continuous variables: geometric interpretations,” Evolution, vol. 55, no. 11, pp. 2143–2160, 2001.
- F. J. Rohlf, “Geometric morphometrics and phylogeny,” in Morphology, Shape, and Phylogeny, N. MacLeod and P.L. Forey, Eds., pp. 175–193, Taylor and Francis, London, UK, 2002.
- E. P. Martins and T. F. Hansen, “Phylogenies and the comparative method: a general approach to incorporating phylogenetic information into the analysis of interspecific data,” American Naturalist, vol. 149, no. 4, pp. 646–667, 1997.
- C. P. Klingenberg, “MorphoJ: an integrated software package for geometric morphometrics,” Molecular Ecology Resources, vol. 11, no. 2, pp. 353–357, 2011.
- A. Ivanovic, N. Tomašević, G. Džukić, and M. L. Kalezić, “Evolutionary diversification of the limb skeleton in crested newts (Triturus cristatus superspecies, Caudata, Salamandridae),” Annales Zoologici Fennici, vol. 45, no. 6, pp. 527–535, 2008.
- P. M. Magwene, “New tools for studying integration and modularity,” Evolution, vol. 55, no. 9, pp. 1734–1745, 2001.
- A. Ivanović, K. Sotiropoulos, T. D. Vukov et al., “Cranial shape variation and molecular phylogenetic structure of crested newts (Triturus cristatus superspecies: Caudata, Salamandridae) in the Balkans,” Biological Journal of the Linnean Society, vol. 95, no. 2, pp. 348–360, 2008.
- S. Steinfartz, S. Vicario, J. W. Arntzen, and A. Caccone, “A Bayesian approach on molecules and behavior: reconsidering phylogenetic and evolutionary patterns of the Salamandridae with emphasis on Triturus newts,” Journal of Experimental Zoology Part B, vol. 308, no. 2, pp. 139–162, 2007.
- R. Griffiths, Newts and Salamanders of Europe, T. Poyser and A. D Poyser, London, UK, 1996.
- T. R. Halliday, “The courtship of European newts. An evolutionary perspective,” in The Reproductive Biology of Amphibians, D. H. Taylor and S. I. Guttman, Eds., pp. 185–232, Plenum, New York, NY, USA, 1977.
- G. E. Themudo, B. Wielstra, and J. W. Arntzen, “Multiple nuclear and mitochondrial genes resolve the branching order of a rapid radiation of crested newts (Triturus, Salamandridae),” Molecular Phylogenetics and Evolution, vol. 52, no. 2, pp. 321–328, 2009.
- J. W. Arntzen and G. P. Wallis, “Geographic variation and taxonomy of crested newts (Triturus cristatus superspecies): morphological and mitochondrial DNA data,” Contribution to Zoology, vol. 68, no. 3, pp. 181–203, 1999.
- J. W. Arntzen and B. Wielstra, “Where to draw the line? A nuclear genetic perspective on proposed range boundaries of the crested newts Triturus karelinii and T. arntzeni,” Amphibia Reptilia, vol. 31, no. 3, pp. 311–322, 2010.
- S. Gavrilets and A. Vose, “Dynamic patterns of adaptive radiation,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 50, pp. 18040–18045, 2005.
- D. Schluter, The Ecology of Adaptive Radiation, Oxford University Press, Oxford, UK, 2000.
- S. Gavrilets and A. Vose, “Dynamic patterns of adaptive radiation: evolution of mating preferences,” in Speciation and Patterns of Diversity, R. K. Butlin, J. R. Bridle, and D. Schulter, Eds., pp. 102–126, Cambridge University Press, Cambridge, UK, 2009.
- T. D. Vukov, K. Sotiropoulos, B. Wielstra, G. Džukić, and M. L. Kalezić, “The evolution of adult body form in the crested newts (Triturus cristatus superspecies, Caudata, Salamandridae),” Journal of Zoological Systematics and Evolutionary Research, vol. 49, no. 4, pp. 324–334, 2011.
- J. Crnobrnja-Isailović, G. Džukić, N. Krstić, and M. L. Kalezić, “Evolutionary and paleogeographical effects on the distribution of the Triturus cristatus superspecies in the central Balkans,” Amphibia Reptilia, vol. 18, no. 4, pp. 321–332, 1997.
- B. Lanza, J. W. Arntzen, and E. Gentile, “Vertebral numbers in the Caudata of the western Palaearctic (Amphibia),” Atti del Museo Civico di Storia Naturale—Trieste, vol. 54, pp. 3–114, 2010.
- R. E. Strauss and R. Altig, “Ontogenetic body form changes in three ecological morphotypes of anuran tadpoles,” Growth, Development and Aging, vol. 56, no. 1, pp. 3–16, 1992.
- N. H. Shubin and P. Alberch, “A morphogenetic approach to the origin and basic organization of the tetrapod limb,” Evolutionary Biology, vol. 20, no. 1, pp. 319–387, 1986.
- L. H. Shubin, “Origin of evolutionary novelty: examples from limbs,” Journal of Morphology, vol. 252, no. 1, pp. 15–28, 2002.
- B. Hallgrímsson, K. Willmore, and B. K. Hall, “Canalization, developmental stability, and morphological integration in primate limbs,” Yearbook of Physical Anthropology, vol. 45, pp. 131–158, 2002.
- N. M. Young and B. Hallgrímsson, “Serial homology and the evolution of mammalian limb covariation structure,” Evolution, vol. 59, no. 12, pp. 2691–2704, 2005.
- R. R. Lawler, “Morphological integration and natural selection in the postcranium of wild Verreaux's sifaka (Propithecus verreauxi verreauxi),” American Journal of Physical Anthropology, vol. 136, no. 2, pp. 204–213, 2008.
- N. M. Young, G. P. Wagner, and B. Hallgrímsson, “Development and the evolvability of human limbs,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 8, pp. 3400–3405, 2010.
- M. Furtula, A. Ivanović, G. Džukić, and M. L. Kalezić, “Egg size variation in crested newts from the western Balkans (Caudata, Salamandridae, Triturus cristatus superspecies),” Zoological Studies, vol. 47, no. 5, pp. 585–590, 2008.
- M. Cvijanović, A. Ivanović, N. T. Kolarov, G. Džukić, and M. L. Kalezić, “Early ontogeny shows the same interspecific variation as natural history parameters in the crested newt (Triturus cristatus superspecies) (Caudata, Salamandridae),” Contributions to Zoology, vol. 78, no. 2, pp. 43–50, 2009.
- R. H. Kaplan, “The implications of ovum size variability for offspring fitness and clutch size within several populations of salamanders (Ambystoma),” Evolution, vol. 34, no. 1, pp. 51–64, 1980.
- F. J. Rohlf and D. Slice, “Extensions of the procrustes method for the optimal superimposition of landmarks,” Systematic Zoology, vol. 39, no. 1, pp. 40–59, 1990.
- F. L. Bookstein, “Combining the tools of geometric morphometrics,” in Advances in Morphometrics, L. F. Marcus, M. Corti, A. Loy, G. J. P. Naylor, and D. E. Slice, Eds., ASI Series A: Life Sciences, Plenum Press, New York, NY, USA, 1996.
- I. L. Dryden and K. V. Mardia, Statistical Shape Analysis, John Wiley & Sons, New York, NY, USA, 1998.
- J. B. Yoder, E. Clancey, S. Des Roches et al., “Ecological opportunity and the origin of adaptive radiations,” Journal of Evolutionary Biology, vol. 23, no. 8, pp. 1581–1596, 2010.
- A. Ivanović, K. Sotiropoulos, M. Furtula, G. Džukić, and M. L. Kalezić, “Sexual size and shape evolution in European newts (Amphibia: Caudata: Salamandridae) on the Balkan Peninsula,” Journal of Zoological Systematics and Evolutionary Research, vol. 46, no. 4, pp. 381–387, 2008.
- S. Gavrilets, H. Li, and M. D. Vose, “Patterns of parapatric speciation,” Evolution, vol. 54, no. 4, pp. 1126–1134, 2000.
- M. Ridley, Evolution, Blackwell Publishing, Malden, Mass, USA, 3rd edition, 2004.
- M. L. Kalezić, D. Cvetković, A. Djorović, and G. Džukić, “Paedomorphosis and differences in life-history traits of two neighbouring crested newt (Triturus carnifex) populations,” The Herpetological Journal, vol. 4, no. 4, pp. 151–159, 1994.
- J. B. Losos, K. I. Warheit, and T. W. Schoener, “Adaptive differentiation following experimental island colonization in Anolis lizards,” Nature, vol. 387, no. 6628, pp. 70–73, 1997.
- D. J. Funk, P. Nosil, and W. J. Etges, “Ecological divergence exhibits consistently positive associations with reproductive isolation across disparate taxa,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 9, pp. 3209–3213, 2006.
- C. Venditti, A. Meade, and M. Pagel, “Phylogenies reveal new interpretation of speciation and the red queen,” Nature, vol. 463, no. 7279, pp. 349–352, 2010.
- M. W. Orr and T. B. Smith, “Ecology and speciation,” Trends in Ecology and Evolution, vol. 13, no. 12, pp. 502–506, 1998.
- S. V. Popov, F. Roegl, A. Y. Rozanov, F. F. Steininger, I. G. Scherba, and M. Kovač, Lithological-Paleogeographic Maps of Paratethys. 10 Maps Late Eocene to Pliocene, Courier Forschungsinstitut Senckenberg, Frankfurt am Main, Germany, 2004.
- S. N. Litvinchuk and L. J. Borkin, Evolution, Systematics, and Distribution of Crested Newts (Triturus cristatus Complex) in Russion and Adjacent Countries, Europeisky Dom, St. Petersburg, Russia, 2009.
- G.P. Wallis and J. W. Arntzen, “Mitochondrial-DNA variation in the crested newt superspecies: limited cytoplasmic gene flow among species,” Evolution, vol. 43, no. 1, pp. 88–101, 1989.
- J. Vörös and J. W. Arntzen, “Weak population structuring in the Danube crested newt, Triturus dobrogicus, inferred from allozymes,” Amphibia Reptilia, vol. 31, no. 3, pp. 339–346, 2010.