Where Do Phosphosites Come from and Where Do They Go after Gene Duplication?
Figure 1
Algorithm used to calculate and compare the proportions of transitions between phosphorylated and phosphomimetic residues relative to control sites. (a) Phosphosite (pS, pT) gains from phosphomimetic amino acids were identified as cases where only one of the paralog has a phosphosite and the ancestral sequence has a phosphomimetic residue at the same position. Control sites (cS, cT) were identified in the same way but considering Ser and Thr that are not known to be phosphorylated. The ancestral sequence was inferred using likelihood or parsimony approaches. Phosphosites losses to phosphomimetic amino acids were identified as cases where one paralog has a phosphosite in a position that is occupied by a phosphomimetic amino acid in the other paralog and a phosphorylatable amino acid at the same position in the ancestral sequence. (b) The proportion of pS or pT that evolved from or to D or E was compared to the proportion of cS or cT that evolved from or to D or E. X represents any amino acid with the exception of Ser, Thr and Tyr.
