Research Article

Microsatellites Cross-Species Amplification across Some African Cichlids

Table 2

Microsatellite loci tested for cross-species amplification with indications of repeat structure observed in O. niloticus (according to Lee and Kocher, [13]), allele size range of the amplified fragment across all tested species, PCR and electrophoresis conditions (labeled primer, annealing temperature/magnesium concentration (mM)/electrophoresis Volt-hour), and amplification quality obtained after PCR optimisation tests (from very good ++ to poor −−; see detail of the categories in main text); loci presenting a wide cross-species amplification efficiency are in bold.

LociGenBank access No.StructureRange (bp)PCR and electrophoresis conditionsAmplification efficiency

UNH-008G31346Perfect196–236R* 56/1.2/6000++
UNH-102G12255Perfect132–185R* 50/1.2/4500++
UNH-103G12256Perfect171–260R* 48/1.2/6000+
UNH-106G12259Compound115–189R* 50/1.2/3500+
UNH-115G12268Compound100–146F* 50/1.5/3500++
UNH-117G12270Interrupted108–146R* 5411.2/4500++
UNH-120G12273CompoundR* 48/2/—− −
UNH-123G12276Perfect142–232F* 48/1.2/4500++
UNH-124G12277Perfect295–324F* 54/1.2/7500++
UNH-125G12278Compound134–198R* 48/1.5/4500+
UNH-129G12282Interrupted180–253R* 48/1.2/4500+
UNH-130G12283Perfect174–242R* 50/1.2/4500+
UNH-131G12284Perfect283–303F* 48/2/6000
UNH-132G12285Perfect100–134R* 52/1.2/3500+
UNH-135G12287Interrupted124–284R* 50/1.5/4500+
UNH-138G12290Perfect144–250R* 48/1.5/4500+
UNH-142G12294Interrupted142–192F* 48/1.2/4500++
UNH-146G12298Interrupted111–149F* 60/1/3500++
UNH-149G12301Perfect143–225R* 48/1.5/4500+
UNH-154G12306Perfect98–176R* 50/1.2/3500++
UNH-159G12311Perfect205–267R* 55/1.2/6000++
UNH-162G12314Perfect125–252R* 48/1.5/6000++
UNH-169G12321Interrupted124–240R* 54/1.2/3500++
UNH-173G12325Perfect124–188F* 55/1.2/4500+
UNH-174G12326Perfect146–187F* 48/1.5/4500++
UNH-189G12341Perfect135–208R* 52/1.2/4500+
UNH-190G12342Compound133–202R* 60/1/4500+
UNH-193G12386PerfectR* 48/2/3500− −
UNH-197G12348Interrupted154–228R* 50/1.2/4500+
UNH-207G12358Interrupted90–198R* 60/1.2/3500++
UNH-211G12362Perfect82–194R* 48/1.5/3500++
UNH-216G12367Perfect126–212R* 52/1.2/3500++