Conservation/Mutation in the Splice Sites of Cytokine Receptor Genes of Mouse and Human

Table 3

Columns 1–4: the nucleotide composition according to the position in the initial hexanucleotides in Mouse and Human. Columns 1 and 5–7: analysis of the conservation of individual nucleotides in the initial hexanucleotides of the introns. Expected random conservation in column 5 and actual conservation in column 6. Percentages are over the total of 216 couples of hexanucleotides.

1	2	3	4	5	6	7
Sequence	% in Mouse	% in Human		Random conservation (%)	Actual conservation (%)
GTAxxx	59.7	57.5		34.03	50.93	𝑃<0.01
GTCxxx	1.9	2.3		0.04	1.85	𝑃<0.01
GTGxxx	37.0	38.4		14.21	30.09	𝑃<0.01
GTTxxx	1.4	2.3		0.03	1.39	𝑃<0.01
Total				48.31	84.26	𝑃<0.01
GTxAxx	82.4	81.0		66.74	77.78	𝑃<0.01
GTxCxx	2.8	2.8		0.08	2.31	𝑃<0.01
GTxGxx	6.9	8.3		0.57	4.17	𝑃<0.01
GTxTxx	7.9	7.9		0.62	6.02	𝑃<0.01
Total				68.01	90.28	𝑃<0.01
GTxxAx	7.9	7.4		0.58	6.48	𝑃<0.01
GTxxCx	1.4	1.8		0.03	0.93	𝑃<0.01
GTxxGx	85.2	84.3		71.82	82.41	𝑃<0.01
GTxxTx	5.5	6.5		0.36	3.70	𝑃<0.01
Total				72.79	93.52	𝑃<0.01
GTxxxA	21.8	24.1		5.25	16.20	𝑃<0.01
GTxxxC	11.6	7.4	𝑃=0.02	0.86	3.70	𝑃<0.01
GTxxxG	16.6	19.9		3.30	8.33	𝑃<0.01
GTxxxT	50.0	48.6		24.30	45.37	𝑃<0.01
Total				33.71	73.6	𝑃<0.01