Individual Genetic Contributions to Genital Shape Variation between <i>Drosophila simulans</i> and <i>D. mauritiana</i>

LeVasseur-Viens, Hélène; Moehring, Amanda J.

doi:https://doi.org/10.1155/2014/808247

International Journal of Evolutionary Biology

On this page

Abstract Introduction Materials and Methods Results Discussion Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2014 | Article ID 808247 | https://doi.org/10.1155/2014/808247

Individual Genetic Contributions to Genital Shape Variation between Drosophila simulans and D. mauritiana

Hélène LeVasseur-Viens¹and Amanda J. Moehring¹

Academic Editor: Yoko Satta

Received11 Jul 2014

Accepted07 Aug 2014

Published08 Sept 2014

Abstract

External genitalia are one of the most rapidly evolving morphological features in insects. In the Drosophila melanogaster species subgroup, males possess a nonfertilizing external genital structure, called the posterior lobe, which is highly divergent among even closely related species. A previous study on this subgroup mapped two genomic regions that affect lobe size and four that affect lobe shape differences between D. mauritiana and D. sechellia; none of the regions affected both size and shape. Here, we investigate whether three of these significant regions also affect lobe size and shape differences between the overlapping species pair D. mauritiana and D. simulans. We found that the same three regions of D. mauritiana, previously shown to affect lobe morphology in a D. sechellia genetic background, also affect lobe morphology in a D. simulans genetic background, with one of the regions affecting both size and shape. Two of the regions also affected morphology when introgressed in the reciprocal direction. The overlap of regions affecting genital morphology within related species pairs indicates either that there is a common underlying genetic basis for variation in genital morphology within this species group or that there are multiple adjacent loci with the potential to influence genital morphology.

1. Introduction

Animal groups ranging from primates [1] to lizards [2] show rapid evolution of male genitalia. In addition to the inseminating, or primary, organs, external secondary organs involved in stimulation and copulation also exhibit rapid divergence in a variety of animal groups [3]. In insects, the divergence of male genitalia is so pronounced that even recently diverged sibling species show a high degree of variation in the male genitalia and/or secondary organs [4–6]. Several different models have been developed to explain the evolution of genitalia in individual species, but none explains why it occurs across so many animal groups [7, 8]. The most prominent competing theories that attempt to explain the pervasive occurrence of rapidly diverging male genitalia are the pleiotropy hypothesis, the lock and key hypothesis, and the sexual selection hypothesis [3, 7, 9–14]. While there is evidence supporting each of these models, sexual selection is thought to be the most prevalent influence on the divergence of male genitalia [3, 15, 16].

An understanding of the genetic underpinnings of genital shape enhances our ability to assess the evolutionary forces influencing genital morphology. One of the most widely used model systems for understanding the genetic basis of genital morphology is the Drosophila melanogaster species subgroup. These species are largely morphologically indistinguishable from one another except for the shape of the male’s exterior genital lobes [17]. The bilaterally symmetrical posterior lobes, also called the genital arch, are a cuticular projection that surrounds the inverted adaegus. The lobes are inserted between the female’s eighth and ninth abdominal tergites during copulation [18] and appear to be involved in several aspects of copulation and fertilization [19–21], making it likely that they experience sexual selection.

Several genetic mapping studies for lobe shape have been performed in this group, and while the maps identify genomic regions and not individual genetic loci, comparisons among studies can significantly enhance our understanding of how these sexually selected traits evolve. Most genetic mapping studies on genital morphology have used quantitative trait locus (QTL) mapping, but a recent study on the sibling species D. mauritiana and D. sechellia [22] used introgression mapping, allowing for the contributions of individual genomic regions to be assessed independently. In Masly et al. [22], small homozygous pieces of the D. mauritiana genome are present in an otherwise homozygous D. sechellia genetic background. They found two genomic regions that caused the size of the lobes to shift closer to that of D. mauritiana, located near the left telomere and the centromere of the third chromosome. They found four other regions that affected lobe shape, including one near the right telomere of the third chromosome. Regions influencing lobe shape did not overlap those found for lobe size. This demonstrated that individual genomic regions could influence genital morphology and that there is a differential genetic basis for the size and shape aspects of genital morphology in this species pair.

Here, we examine whether the ability of individual genomic regions to influence lobe morphology and the genetic uncoupling of size and shape is present in other species pairs or if it is unique to the D. mauritiana-D. sechellia species pair. We performed this study in the overlapping species pair of D. simulans-D. mauritiana, allowing us to additionally examine whether the same loci would underlie genital morphology differences in related sibling species or if each species in this subgroup owes its unique morphology to separate loci. The sister species D. simulans and D. mauritiana have been a well-studied example of genital morphology divergence within this subgroup [5, 18, 19, 23]: D. simulans males have helmet-shaped lobes, while D. mauritiana males have stick-like protrusions [5, 22, 24]. When these two species are crossed, hybrid males have an intermediate posterior lobe morphology when compared to the two parental species, while males resulting from a backcross to either parent species produce a continuous range of lobe phenotypes [5, 23], indicating a polygenic nature for lobe morphology, which has been confirmed by QTL mapping [23]. Although genomic regions were located using QTL mapping, it is unknown whether they individually will have an effect on male genital morphology. Indeed, since none of the individual regions had a large effect on the phenotype, it is possible that the effect of a single locus might be undetectable when it is measured individually.

We utilize introgression lines to assess the contributions of individual genomic regions to the divergent genital lobe size and shape between D. simulans and D. mauritiana. We focused on the three regions identified in Masly et al. [22] on the third chromosome as individually influencing D. mauritiana and D. sechellia lobe morphology: left telomere, centromere, and right telomere. Previous QTL mapping studies identified these same three regions as contributors to lobe morphology in the D. simulans-D. mauritiana species pair [5, 23]. Since the previous work on genital morphology [22] found that some regions of the genome affected the lobes in a direction opposite to expectation (increasing size when they should have decreased size), presumably due to transgressive segregation arising from either additive effects or epistatic interactions with the genetic background, we assess introgressions in both directions: lines that are entirely D. mauritiana except for an introgressed D. simulans genomic segment (M_S) and the reciprocal lines that are entirely D. simulans except for a D. mauritiana introgression (S_M). We compared the lobe size and shape of these lines to the lobes of the species contributing the majority of the genomic complement and evaluated whether the introgressed genome affected lobe size and/or shape.

2. Materials and Methods

2.1. Drosophila Stocks

Introgression lines for the third chromosome were previously created [25] by repeated backcrossing of F₁ hybrids to their parent species and then by several generations of brother-sister mating, paired with molecular genotyping at every generation, to make the introgressions homozygous. Genetic markers were then used to determine the location of the genomic region of the opposite species. The resulting introgression lines of D. simulans and D. mauritiana contain known inserted regions of the opposite species within their respective genomes (Table 1).

Introgression lines containing each of the three cytological locations important for posterior lobe morphology (left telomere, centromere, and right telomere) were used for dissections of the posterior lobe; we assayed the lines containing the largest introgressions in these regions to increase the likelihood of capturing genetic factors for genital morphology (Table 1) [25]. We have maintained the nomenclature used in McNiven and Moehring [25] for consistency. The three backcrossed D. mauritiana lines with known introgressed D. simulans genomic regions (M_S) were line (containing the left telomeric region from D. simulans, near cytological band 62), line (containing the centromeric region from D. simulans, near cytological band 82), and line (containing the right telomeric region from D. simulans, near cytological band 98). The five backcrossed D. simulans lines with known D. mauritiana genomic regions dissected were lines and (containing the D. mauritiana left telomeric region), line (containing the D. mauritiana centromeric region), and lines and (containing the D. mauritiana right telomeric region).

2.2. Comparing Posterior Lobe Area, Length, and Width

A microknife was used to remove one randomly chosen posterior lobe from the abdomen in TE buffer. A coverslip was then used to ensure that the posterior lobe was observed in a single focal plane. An E100 Nikon compound microscope equipped with a 5-megapixel camera was used to visualize the posterior lobes. All lobe measurements were performed using the computer software NIS-Elements 3.1 (sample size ). Lengths were measured in D. simulans as the distance from the base of the lobe to the furthest vertical point, as drawn by a line perpendicular to the base; in D. mauritiana, the length was measured from the midpoint of the baseline to the furthest point (Figure 1). We found that these two different measures of length in the two species were necessary in order to obtain consistent results due to the general lack of morphological landmarks on the lobes. Widths were measured along a horizontal line at the widest point of the lobe; area was measured by outlining the perimeter of the lobe (Figure 1). All values were first corrected for body size using the tibia length measurements prior to statistical comparison. A one-way ANOVA was used to determine if there was a significant difference in the area, length, or width of the posterior lobes, when comparing the introgressed lines to the parental species comprising the genetic background.

(a)

(b)

(c)

(d)

2.3. Elliptical Fourier Analysis and Principal Component Analysis

Due to the paucity of morphometric landmarks, an elliptical Fourier analysis was used to represent each posterior lobe’s shape () [5]. We were able to accomplish this because of the 2D nature of the posterior lobe. To do this, the SHAPE program [26] was used to first normalize the posterior lobe shape of males from introgression lines by the area of the lobe in order to correct for size differences and assign a chaincode value. Chaincode is a coding system within the SHAPE software for representing geometrical shapes as numbers. These values were then used to calculate the elliptical Fourier descriptors (EFD) and to visualize them for comparisons. We obtained 20 Fourier harmonics per posterior lobe, which allowed for precise outlines.

To determine how many variables could be used to explain the variation between the introgression lines compared to the wild type lines, a principal components analysis (PCA) [5] was performed, also using the SHAPE program, for each backcross type. The PCA performed using PrinComp, a component of the SHAPE program, is based on the variance-covariance matrix. In both the D. simulans and D. mauritiana, PC1–PC7 explained at least 90% of the variation observed when comparing introgression lines to the pure-species lines. PC1 and PC2 were evaluated separately using a single-factor ANOVA for differences between the introgression line’s genital lobe shape and the lobe shape of the parental species that contributed the genetic background.

3. Results

3.1. Comparison of Posterior Lobes due to D. mauritiana Introgressions

When comparing the overall morphology of the posterior lobes of the introgression lines, the morphology appeared to be species-specific and predominantly in accordance with the backcross genetic background (Table 2; Figure 2). Lobe area showed a strong correlation with both lobe width (values from 0.64 to 0.93) and lobe length (values from 0.50 to 0.80), with a stronger correlation for lobe width in all lines. Posterior lobes in the parental D. simulans males were significantly wider and longer and had a greater mean area when compared to the posterior lobes of males containing the D. mauritiana introgression in line (; , , and , resp.). The introgression line from the same region, , also displayed reduced lobe size [lobe area (mm²)/tibia size (mm) = ()], but the lobes appeared to be aberrant and malformed in some of the dissections performed (2/10). These sporadic differences observed in the one line are unlikely to be due to the species-specific introgression as they were not observed in the overlapping line . As such, we removed this line from further analyses. However, if the observed differences were due to the introgression, then the loci for shape would fall within the small region of unknown genotype on the border between the markers assessed as being the introgression versus parental genotype.

(a)

(b)

(c)

Figure 2

Comparison of posterior lobes in introgression lines versus parental lines. Box plots show the distribution of the uncorrected values for area (a), width (b), and length (c) of the posterior lobes in D. simulans FC (sim; grey boxes), introgressions of D. mauritiana into a D. simulans genetic background (S_M; grey), introgressions of D. simulans into a D. mauritiana genetic background (M_S; white), and D. mauritiana SYN (mau; white). Boxes represent the interquartile range, with the inner horizontal line at the median and the vertical lines denoting the maximum and minimum values (). The introgression lines that were significantly different from the parental line constituting the genetic background, after correction for body size, are marked with , , and . Note that, to allow for comparison to Masly et al. [22], the box plot values in the figure are uncorrected for body size; statistical significance, however, was calculated on values that were corrected for body size.

Significantly greater width and area were also observed for the introgression line (; , , resp.). The length and area of the posterior lobe were also significantly different when comparing the posterior lobes of parental D. simulans males to those from the introgression line (, , ) and approached significance for width (, ). The posterior lobes from the partially overlapping introgression line did not differ significantly in mean width, length, or area when compared to the posterior lobes of parental D. simulans. It should be noted that, for practical reasons, we used a slightly different protocol for measuring length in D. simulans males than in D. mauritiana males, and this may have biased our results for this phenotype. However, since the lobes of introgression males largely resembled those of the parental species comprising the genetic background, these different measures likely had a minor, if any, effect on our assessment of length in introgression males. None of the introgression lines had a significant difference in tibia length compared to pure-species D. mauritiana. There was a slightly negative, and nonsignificant, correlation between individual measures of lobe area and tibia length (, ).

In the principal component analysis, PC1-9 accounted for 95.0% of the variance in the S_M lines, with the majority of the variance explained by PC1 (35.0%) and PC2 (21.4%). PC1, as expected, largely indicated differences in lobe area. Comparisons of PC1 and PC2 between the introgression lines and the parental D. simulans line identified which introgressed regions affected the species-specific shape of the posterior lobe (Figure 3(a)). The shape of the posterior lobe was not significantly affected by an introgressed region near the left telomere in line . However, the introgressed region at the centromere (in line ) significantly affected both PC1 (, , ) and PC2 (, , ), while both lines containing an introgressed segment at the right telomere ( and ) had a significant difference in PC1 (, , ; , , , resp.) but not in PC2.

(a)

(b)

Figure 3

Shape measurements in introgression lines. The distribution of the first two principal components obtained from an elliptical Fourier analysis for pure-species D. mauritiana (green triangles) and D. simulans (orange squares) compared to introgression lines (blue diamonds) containing (a) introgressed regions of D. mauritiana in an otherwise D. simulans genetic background (S_M) or (b) introgressed regions of D. simulans in an otherwise D. mauritiana genetic background (M_S). Ellipses represent the standard deviation centered on the mean value for each group. A representative lobe shape for each line is shown in the same color as the group it represents.

3.2. Comparison of Posterior Lobes due to D. simulans Introgressions

As with the above introgressions, the overall morphology of the posterior lobes in lines with an introgression from D. simulans was species-specific and predominantly similar to that of the parental genetic background, D. mauritiana. In contrast to what was seen for S_M lines, the M_S lines showed generally weaker and more variable correlations between lobe area and lobe width or length: (width: 0.72, length: 0.65), (width: 0.31, length: 0.28), and (width: 0.44, length: 0.51). There was a significant difference in the mean width and area of the posterior lobe when comparing the parental D. mauritiana to the introgression line (; , , resp.). The introgression lines from the other two cytological locations, and , did not show any statistically significant difference in mean width, length, or area of the posterior lobe when compared to those of the parental D. mauritiana males, but did approach significance for width (, ). As with the D. mauritiana introgression males, our different protocol for length measurements in the two parental species may have biased our results, but this is unlikely. None of the introgression lines had a significant difference in tibia length compared to pure-species D. simulans, and there was a nonsignificant negative correlation between lobe area and tibia length (, ).

In the principal component analysis, PC1-9 accounted for 95.6% of the variance in the M_S lines, with the majority of the variance explained by PC1 (41.0%) and PC2 (24.8%). As with the S_M introgressions, PC1 for the M_S lines largely indicated differences in lobe area. Comparisons of PC1 and PC2 between the introgression lines and the parental D. mauritiana lines found that regions at the left telomere and centromere affected the species-specific shape of the posterior lobe (Figure 3(b)). Line , which has an introgression at the left telomere, significantly differed in shape for PC1 (, , ) but not for PC2. Line , with an introgression at the centromere, was significantly different in shape for both PC1 (, , ) and PC2 (, , ), while line , with an introgression at the right telomere, did not significantly differ in either aspect of shape.

4. Discussion

The Drosophila melanogaster subgroup is highly divergent with regard to the shape of the male posterior lobe. Aside from the posterior lobe, there are no other significant differences in overall body morphology between the species, and there is very little correlation between body size and lobe size or shape [5, 22, 27, 28], although lobe size was found to be correlated with tibia length in a recent study [29]. The differences in genital morphology are caused by multiple genomic regions that, in general, act additively to contribute to the shape and size of the posterior lobe [5, 22, 23, 28, 30]. A previous study that utilized quantitative trait locus (QTL) mapping found that the genetic regions that determine size and shape differences between the posterior lobes of D. simulans and D. mauritiana were indistinguishable [5], and therefore lobe, size, and shape were considered genetically linked in these species. In contrast, a study utilizing introgression lines found that the genomic regions influencing the species-specific difference in size for the lobes of D. mauritiana compared to D. sechellia were often different from those that conferred differences in lobe shape [22], indicating that differences in lobe size and shape in these species have separate genetic bases. Our findings agree with both of these previous studies: some regions of the genome contribute to both size and shape, while others affect either size or shape (Figures 4(a) and 4(b)). Thus, there is genetic linkage (association, physical linkage, or pleiotropy) between some loci influencing size and shape differences between D. simulans and D. mauritiana, while other loci either are not linked or are linked with loci whose effect is too small to be detected in this study.

Figure 4

Genomic regions that significantly affect species-specific lobe size and shape. The third chromosome is shown at the top of the figure: the black circle is the centromere, and the brackets represent a fixed inversion difference between D. melanogaster compared to D. simulans, D. mauritiana, and D. sechellia. Numbers above the chromosome are approximate cytological regions. (a) Introgressions of D. mauritiana into an otherwise D. simulans genetic background (S_M) and (b) introgressions of D. simulans into an otherwise D. mauritiana genetic background (M_S) that significantly affected the area (filled red bars; data from Table 2, Figure 2) or shape (filled blue bars; data from Figure 3) of the posterior lobe; open bars indicate nonsignificance for these traits. The length of the bars approximates the size of the introgressed segment. (c) Introgressions of D. mauritiana into an otherwise D. sechellia genetic background (adapted from [22]) that significantly affected the area (red circles) or shape (blue circles) of the posterior lobe. The circles represent the midpoint of the introgression; there is a fade-out on the periphery to indicate that the boundaries of these introgressions are not published. All of the introgressions in (a), (b), and (c) are aligned with their representative cytological positions on the chromosome.

When portions of the D. mauritiana genome were introgressed into D. sechellia, introgressions at the left telomere and centromere influenced size, while an introgression at the right telomere altered the shape of the D. sechellia lobe towards a D. mauritiana-like appearance (Figure 4(c)) [22]. We found that these three regions of D. mauritiana have the same effect on D. simulans lobe morphology as the one they have on D. sechellia lobe morphology, with an additional effect on shape for the centromeric region; this additional effect is likely due to the large size of this introgression (Figures 4(a) and 4(c)), as the significant introgression into D. sechellia does not span the entire length of the genomic region we introgressed into D. simulans ([22]; J. P. Masly, personal communication). One of the three regions (at the centromere) was also implicated as contributing to intraspecific variation in lobe morphology within D. melanogaster [30]. It is therefore possible that there may be a similar genetic underpinning for genital divergence in this species group; this is not surprising, as it makes sense that the same developmental pathways could be influenced by selection during these species’ divergence.

Only one of the three regions had the same effect on lobe morphology when they were introgressed in the alternate direction; that is, D. simulans genome introgressed into D. mauritiana (Figure 4(b)). For example, the introgression at the left telomere affects lobe shape rather than size, demonstrating that the genes in this region likely do not have the same effect on the two species as the portion of the genome that is introgressed in is also present in line . Likewise, the introgression , which does not have an effect on lobe morphology, contains all of the equivalent genomic regions present in the significant lines and and contains all of the regions present in the D. mauritiana introgression that significantly affected lobe shape in D. sechellia ([22]; J. P. Masly, personal communication). Thus, there is divergence in how individual genomic regions influence morphology, and the loci within these regions appear to interact with their genetic background. Two of these regions, although they had a significant effect on shape () or area and shape () in our study, do not directly overlap the location of the introgressions of D. mauritiana into D. sechellia that were shown to have a significant effect on lobe area ([22]; J. P. Masly, personal communication). Thus, it appears that there may be regions of the genome that harbor multiple loci that have the potential of contributing to the variation in lobe morphology in this species group.

Although they were not identified as significantly influencing lobe area in the D. mauritiana-D. sechellia species pair, these regions were found to influence lobe phenotype, but in an unexpected direction [22]. The introgression 2H3(B) overlaps the same region covered here by , but in the former study the introgressed piece of D. mauritiana caused the lobe to have a larger size than either parental species and skewed the shape away from that of D. mauritiana. Likewise, introgression 2K3(A) overlaps but increased the size above that of either parental species. This skew in lobe phenotype away from the expected direction (i.e., the phenotype became even more dissimilar from D. mauritiana) was most likely due to epistatic interactions or transgressive segregation [22]. In contrast, none of our introgressions produced a significant phenotype in the opposite direction to the expected in either size (Table 1, Figure 2) or shape (Figure 3). Thus, it appears that the observed skew due to introgressions of D. mauritiana for these regions was due to their placement into a D. sechellia genetic background; when they are placed into a D. simulans genetic background, they significantly affect size and/or shape in the expected direction.

As was found in the D. mauritiana-D. sechellia species pair [22], our results also indicate that single genomic regions can significantly modify genital morphology, suggesting that individual genes may have a strong enough effect on lobe morphology that it may be possible to map their separate locations. This result is still somewhat unexpected as lobe morphology in the D. simulans-D. mauritiana species pair was previously mapped to more than 19 genomic regions [23], making future fine-mapping appear impossible as each region was assumed to have too small of an effect to be individually detectable by reasonable means. While this still may be the case, as our introgressed regions are large and may harbor multiple loci of small effect, the lobe area shifted by 22–24% in our significant lines, making the phenotype relatively pronounced, enhancing the prospect of future fine-mapping.

The region at the right telomere that was significant for both D. simulans-D. mauritiana and D. mauritiana-D. sechellia lobe shape harbors a candidate gene for posterior lobe morphology [22, 31]. The D. melanogaster gene known as Drop (Dr), at cytological location 99B, has been identified as important in sex determination. Dr is repressed in females during development and, when mutated in D. melanogaster males, leads to misshapen posterior lobes [31]. A comparison of published sequences [32] confirmed that there is a homolog for Dr in both D. simulans and D. mauritiana in the same cytological region, making this gene a strong candidate for variation in lobe morphology in this species pair.

The posterior lobes are thought to play a role in both copulation and fertilization [19–21], and as such, divergence in lobe morphology could influence male mating success with females of another species. The same telomeric and centromeric regions on the 3rd chromosome that affect genital shape morphology here have also been found to affect mating behavior in D. simulans-D. mauritiana [25, 33] and D. simulans-D. melanogaster [34]. We can examine whether the different lobe morphology induced by the introgressions has an impact on mating behavior by testing the behavior of the introgression lines. A previous study examined three of the same introgression lines used here for their effect on male mating success [25]. When D. mauritiana males harboring a D. simulans introgression were paired with D. mauritiana females, the males with an introgression at the centromere () and right telomere () had a significant reduction in copulation success, while males with an introgression at the left telomere () did not have reduced mating success. As these results do not align with our significant results for alteration in lobe size or shape (Figure 4(b)), differences in male mating success do not appear to be induced by the variation in lobe morphology observed for these lines, but additional tests are required to rule out linkage between these traits. Additionally, genes for a sexually selected trait are again found to localize near the centromere and telomeres, a trend that is potentially widespread [34].

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Acknowledgments

The authors want to thank Ben Rubin for his assistance with data analysis and John Masly for his helpful comments on the paper. This work was funded by the Canada Research Chairs program and a National Science and Engineering Research Council Discovery grant to Amanda J. Moehring.

References

A. F. Dixson, “Sexual selection, genital morphology, and copulatory behavior in male Galagos,” International Journal of Primatology, vol. 10, no. 1, pp. 47–55, 1989.
View at: Publisher Site | Google Scholar
W. Böhme and T. Ziegler, “A review of iguanian and anguimorph lizard genitalia (squamata: Chamaeleonidae; Varanoidea, shinisauridae, xenosauridae, anguidae) and their phylogenetic significance: comparisons with molecular data sets,” Journal of Zoological Systematics and Evolutionary Research, vol. 47, no. 2, pp. 189–202, 2009.
View at: Publisher Site | Google Scholar
W. G. Eberhard, Sexual Selection and Animal Genitalia, Harvard University Press, Cambridge, Mass, USA, 1985.
O. Richards, “The specific characters of the British humblebees (Hymenoptera),” Transactions of the Entomological Society of London, vol. 75, pp. 233–268, 1927.
View at: Google Scholar
J. Liu, J. M. Mercer, L. F. Stam, G. C. Gibson, Z.-B. Zeng, and C. C. Laurie, “Genetic analysis of a morphological shape difference in the male genitalia of Drosophila simulans and D. mauritiana,” Genetics, vol. 142, no. 4, pp. 1129–1145, 1996.
View at: Google Scholar
H. Song, “Species-specificity of male genitalia is characterized by shape, size, and complexity,” Insect Systematics & Evolution, vol. 40, no. 2, pp. 159–170, 2009.
View at: Publisher Site | Google Scholar
G. Arnqvist and R. Thornhill, “Evolution of animal genitalia: patterns of phenotypic and genotypic variation and condition dependence of genital and non-genital morphology in water strider (Heteroptera: Gerridae: Insecta),” Genetical Research, vol. 71, no. 3, pp. 193–212, 1998.
View at: Publisher Site | Google Scholar
L. W. Simmons, C. M. House, J. Hunt, and F. García-González, “Evolutionary response to sexual selection in male genital morphology,” Current Biology, vol. 19, pp. 1442–1446, 2009.
View at: Google Scholar
W. G. Eberhard, “Animal genitalia and female choice,” American Scientist, vol. 78, pp. 134–141, 1990.
View at: Google Scholar
W. G. Eberhard, “Species isolation, genital mechanics, and the evolution of species- specific genitalia in three species of Macrodactylus beetles (Coleoptera, Scarabeidae, Melolonthinae),” Evolution, vol. 46, no. 6, pp. 1774–1783, 1992.
View at: Google Scholar
J. P. Masly, “170 years of “Lock-and-Key”: genital morphology and reproductive isolation,” International Journal of Evolutionary Biology, vol. 2012, Article ID 247352, 10 pages, 2012.
View at: Publisher Site | Google Scholar
D. J. Hosken and P. Stockley, “Sexual selection and genital evolution,” Trends in Ecology and Evolution, vol. 19, no. 2, pp. 87–93, 2004.
View at: Publisher Site | Google Scholar
G. Arnqvist, “The evolution of water strider mating systems: causes and consequences of sexual conflicts,” in The Evolution of Mating Systems in Insects and Arachnids, pp. 146–163, Cambridge University Press, Cambridge Books Online, Cambridge, UK, 1997.
View at: Google Scholar
A. M. Shapiro and A. H. Porter, “The lock-and-key hypothesis: evolutionary and biosystematic interpretation of insect genitalia,” Annual Review of Entomology, vol. 34, pp. 231–245, 1989.
View at: Google Scholar
W. G. Eberhard, “Evaluating models of sexual selection: genitalia as a test case,” The American Naturalist, vol. 142, pp. 564–571, 1993.
View at: Google Scholar
W. G. Eberhard, “Evolution of genitalia: theories, evidence, and new directions,” Genetica, vol. 138, no. 1, pp. 5–18, 2009.
View at: Publisher Site | Google Scholar
J. A. Coyne, “Genetic basis of differences in genital morphology among three sibling species of Drosophila,” Evolution, vol. 37, pp. 1101–1118, 1983.
View at: Google Scholar
H. M. Robertson, “Mating asymmetries and phylogeny in the Drosophila melanogaster species complex,” Pacific Science, vol. 42, pp. 72–80, 1988.
View at: Google Scholar
J. A. Coyne, “The genetics of an isolating mechanism between two sibling species of Drosophila,” Evolution, vol. 47, pp. 778–788, 1993.
View at: Google Scholar
C. S. C. Price, C. H. Kim, C. J. Gronlund, and J. A. Coyne, “Cryptic reproductive isolation in the Drosophila simulans species complex,” Evolution, vol. 55, no. 1, pp. 81–92, 2001.
View at: Publisher Site | Google Scholar
S. Jagadeeshan and R. S. Singh, “A time-sequence functional analysis of mating behaviour and genital coupling in Drosophila: role of cryptic female choice and male sex-drive in the evolution of male genitalia,” Journal of Evolutionary Biology, vol. 19, no. 4, pp. 1058–1070, 2006.
View at: Publisher Site | Google Scholar
J. P. Masly, J. E. Dalton, S. Srivastava, L. Chen, and M. N. Arbeitman, “The genetic basis of rapidly evolving male genital morphology in Drosophila,” Genetics, vol. 189, no. 1, pp. 357–374, 2011.
View at: Publisher Site | Google Scholar
Z.-B. Zeng, J. Liu, L. F. Stam, C.-H. Kao, J. M. Mercer, and C. C. Laurie, “Genetic architecture of a morphological shape difference between two Drosophila species,” Genetics, vol. 154, no. 1, pp. 299–310, 2000.
View at: Google Scholar
H. M. Robertson, “Mating behavior and the evolution of Drosophila mauritiana,” Evolution, vol. 37, pp. 1283–1293, 1983.
View at: Google Scholar
V. T. K. McNiven and A. J. Moehring, “Identification of genetically linked female preference and male trait,” Evolution, vol. 67, no. 8, pp. 2155–2165, 2013.
View at: Publisher Site | Google Scholar
H. Iwata and Y. Ukai, “SHAPE: a computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors,” The Journal of Heredity, vol. 93, no. 5, pp. 384–385, 2002.
View at: Publisher Site | Google Scholar
J. A. Coyne, J. Rux, and J. R. David, “Genetics of morphological differences and hybrid sterility between Drosophila sechellia and its relatives,” Genetical Research, vol. 57, no. 2, pp. 113–122, 1991.
View at: Publisher Site | Google Scholar
S. J. Macdonald and D. B. Goldstein, “A quantitative genetic analysis of male sexual traits distinguishing the sibling species Drosophila simulans and D. sechellia,” Genetics, vol. 153, no. 4, pp. 1683–1699, 1999.
View at: Google Scholar
C. M. House, Z. Lewis, D. J. Hodgson et al., “Sexual and natural selection both influence male genital evolution,” PLoS ONE, vol. 8, no. 5, Article ID e63807, 2013.
View at: Publisher Site | Google Scholar
C. L. McNeil, C. L. Bain, and S. J. Macdonald, “Multiple quantitative trait loci influence the shape of a male-specific genital structure in Drosophila melanogaster,” G3: Genes, Genomes, Genetics, vol. 1, no. 5, pp. 343–351, 2011.
View at: Publisher Site | Google Scholar
S. S. Chatterjee, L. D. Uppendahl, M. A. Chowdhury, P.-L. Ip, and M. L. Siegal, “The female-specific Doublesex isoform regulates pleiotropic transcription factors to pattern genital development in Drosophila,” Development, vol. 138, no. 6, pp. 1099–1109, 2011.
View at: Publisher Site | Google Scholar
S. J. Marygold, P. C. Leyland, R. L. Seal et al., “FlyBase: improvements to the bibliography,” Nucleic Acids Research, vol. 41, no. 1, pp. D751–D757, 2013.
View at: Publisher Site | Google Scholar
A. J. Moehring, J. Li, M. D. Schug et al., “Quantitative trait loci for sexual isolation between Drosophila simulans and D. mauritiana,” Genetics, vol. 167, no. 3, pp. 1265–1274, 2004.
View at: Publisher Site | Google Scholar
M. Laturney and A. J. Moehring, “The genetic basis of female mate preference and species isolation in Drosophila,” International Journal of Evolutionary Biology, vol. 2012, Article ID 328392, 13 pages, 2012.
View at: Publisher Site | Google Scholar

Copyright

Copyright © 2014 Hélène LeVasseur-Viens and Amanda J. Moehring. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PDF Download Citation

Download other formats

Order printed copies

Views

2285

Downloads

460

Citations