Research Article
Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp
Table 1
The SIA operating sequence for sulfonamides analysis.
| | Step | Syringe pump Action | Position SL | Position SW | Volume (mL) | Flow rate (mL min−1) |
| | (1) Column condition | (a) Aspirate | 4 | | 1 | 3 | | (b) Dispense | 8 | | 1 | 0.6 | | (2) Sample loading | (a) Aspirate | 1 | | 1 | 3 | | (b) Dispense | 8 | | 1 | 0.6 | | (3) Washing | (a) Aspirate | 3 | | 1 | 3 | | (b) Dispense | 8 | Loading | 1 | 0.6 | | (4) Elution | (a) Aspirate | 2 | 0.24 | 3 | | (b) Dispense | 8 | | 0.24 | 0.6 | | (5) Column cleaning | (a) Aspirate | 2 | | 5 | 3 | | (b) Dispense | 8 | | 5 | 0.6 | | (c) Aspirate | 3 | | 2.5 | 3 | | (d) Dispense | 8 | | 2.5 | 0.6 |
| | (6) Injection | HPLC pump | — | Injection | — | 1.5 |
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