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International Journal of Engineering Mathematics

Volume 2013 (2013), Article ID 132827, 8 pages

http://dx.doi.org/10.1155/2013/132827

## Mathematical Modeling and Analysis of the Kinetics of Thermal Inactivation of Enzyme

^{1}Department of Mathematics, The Madura College, Madurai, Tamilnadu 625011, India^{2}Department of Mathematics, Sri Ramakrishna Institute of Technology, Coimbatore, Tamilnadu 641010, India

Received 22 March 2013; Accepted 4 July 2013

Academic Editor: Yurong Liu

Copyright © 2013 S. P. Ananthi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

#### Abstract

A theoretical model of Illeova et al. (2003) thermal inactivation of urease is discussed. Analytical expressions pertaining to the molar concentrations of the native and denatured enzyme are obtained in terms of second-order reaction rate constant. Simple and closed form of theoretical expression pertains to the temperature are also derived. In this paper, homotopy analysis method (HAM) is used to obtain approximate solutions for a nonlinear ordinary differential equation. The obtained approximate result in comparison with the numerical ones is found to be in satisfactory agreement.

#### 1. Introduction

Urease is a good catalyst for the hydrolysis of urea. Several excellent techniques are available to assess urease activity [1, 2]. In 1926, urease was isolated by Summner from the seeds of jack bean as a pure, crystalline enzyme [3]. These crystals, the first obtained for a known enzyme, played a decisive role in proving the protein nature of enzymes. Approximately 50 years later, jack bean urease was identified as the first nickel metalloenzyme [4]. A method for the determination of mercury (II) ions at trace levels is described. The method is based on the profound inhibitory effect of mercury on the enzyme urease [5]. For unknown reasons some seeds are particularly rich sources of urease, and this enzyme has been extensively studied in seeds of various Leguminosae, Cucurbitaceae, Asteraceae and Pinaceae [6, 7]. Jack bean urease, which is the most widely used plant urease, is a nickel containing oligomeric enzyme exhibiting a high degree of specificity to urea [8]. Numerous papers have been published on the applications of urease in biotechnology, including the determination of urea for analytical and biomedical purposes and analysis of heavy metal content in natural drinking water and surface water [9]. Hirai et al. [10] study the structural change of jack bean urease induced by addition of synchrotron radiation. Lencki et al. [11] discuss the effect of subunit dissociation, denaturation, aggregation, coagulation, and decomposition on enzyme inactivation kinetics. Omar and Beauregard [12] investigate the unfolding of jack bean urease by fluorescence emission spectroscopy.

To our knowledge, no rigorous analytical expressions of molar concentrations of the native enzyme, denatured enzyme, and temperature for thermal inactivation of urease for the parameters , , , , , , and have been reported. The purpose of this communication is to derive simple approximate analytical expression for the nonsteady-state concentrations for thermal inactivation of urease using Homotopy analysis method.

#### 2. Mathematical Formulation of Analysis and Problems

Equation (1) illustrates a three-step mechanism of inactivation with a dissociation reaction of the native form of the enzyme, N, into a denatured form, D, and with two parallel association reactions of the native and denatured forms into irreversibly denatured enzyme forms and , respectively: where , , , and represent the rate constants of individual reactions. The material balances equations for N and D and temperature are given as follows [1]: where and are the molar concentrations of the native and denatured enzyme form and is the temperature. The initial boundary condition is

#### 3. Concentrations of Native Enzyme Form, Denatured Enzyme Form, and Temperature under Nonsteady State Condition

A powerful, easy-to-use analytic tool for nonlinear problems in general, namely, the homotopy analysis method. This analytic solution agrees well with numerical results and can be regarded as a definition of the solution of the considered nonlinear problem [13]. The homotopy analysis method (HAM) provides an analytical solution in terms of an infinite power series. In order to investigate the accuracy of the solution with a finite number of terms, the system of differential equations was solved. This method is a good technique when comparing to perturbation method and the numerical simulation. Homotopy analysis method has many advantages since it doesn’t rely on a special small parameter [14]. The results reveal that the method is very effective and simple. The method contains the auxiliary parameter *ℏ*, which provides us with a simple way to adjust and control the convergence region of solution series [15, 16]. Homotopy analysis method provides a powerful tool to strongly analyze linear and nonlinear problems [17, 18]. In this paper, homotopy analysis method (see Appendix A) is used to solve nonlinear differential equations. The analytical expression of molar concentrations of N, D, and temperature (see Appendices A and B) are
These equations represent the new analytical expression of the molar concentrations of the native enzyme, denatured enzyme, and temperature for various values of the parameters , , , , , , and .

#### 4. Numerical Simulation

To show the efficiency of the present method, our nonsteady-state result is compared with numerical solution. Equations (4) and (5) are also solved by numerical method using Matlab/Scilab program. The Scilab/Matlab program [19] is also given in Algorithm 1. The numerical values of parameters employed in Illeova et al. [1] and in this study are given in Table 1. The numerical solution is compared with our analytical result in Figures 1(a)–1(e).

#### 5. Discussions

Equations (4) and (5) represent the closed form of an analytical expression of concentrations of native and denatured enzyme for all values of parameters. In Figures 1(a)–1(e), the analytical results are compared with numerical results for various values of parameters and satisfactory agreement is noted. Figures 1(a)–1(d) represent the molar concentrations for various values of rate constant. From the figure, it is inferred that, when time increases the concentration of decreases whereas the concentration of increases. The time taken to reach the maximum value of is the same as the time taken to reach the minimum value of . The steady value of and depends upon the rate constants.

Figures 2(a)–2(c) represent the molar concentration of N versus time for various values of rate constant and . From that figure, it is observed that, increases when and increase. Figure 3 represents the molar concentration of D versus time for various values of parameter . From this figure, it is found that, the value of concentration initially increases and reaches the steady state value when . The concentration increases when increases. The concentration becomes zero when s^{−1}. Figure 1(e) denotes the representation temperature versus time for various values of . The value of temperature increases when increases. It is almost linear with respect to time .

#### 6. Conclusion

In this work, we obtained the analytical expression of concentrations in terms of rate constants , , , , bath temperature , and coefficient in the enthalpy balance . The nonlinear ordinary differential equations have been solved analytically. The closed analytical expressions of molar concentrations of , , and temperature are obtained using the homotopy analysis method. An agreement with the numerical result is noted. The information gained from this theoretical model can be useful for the kinetic analysis of the experimental results over handling rate constants and molar concentrations.

#### Appendices

#### A. Basic Concept of Homotopy Analysis Method

Consider the following differential equation [20–22]: where is a nonlinear operator, denotes an independent variable, and is an unknown function. For simplicity, we ignore all boundary or initial conditions, which can be treated in a similar way. By means of generalizing the conventional homotopy method, Liao [19] constructed the so-called zero-order deformation equation as follows: where is the embedding parameter, is a nonzero auxiliary parameter, is an auxiliary function, is an auxiliary linear operator, is an initial guess of , and is an unknown function. It is important that one has great freedom to choose auxiliary unknowns in HAM. Obviously, when and , it holds the following: respectively. Thus, as increases from 0 to 1, the solution varies from the initial guess to the solution . Expanding in Taylor series with respect to , we have where If the auxiliary linear operator, the initial guess, the auxiliary parameter , and the auxiliary function are so properly chosen and the series (A.4) converges at , then we have Differentiating (A.2) for times with respect to the embedding parameter , and then setting and finally dividing them by , we will have the so-called th-order deformation equation as follows: where Applying on both sides of (A.7), we get In this way, it is easily to obtain for , at th order, we have When , we get an accurate approximation of the original equation (A.1). For the convergence of the above method, we refer the reader to Liao [13]. If (A.1) admits unique solution, then this method will produce the unique solution. If (A.1) does not possess unique solution, the HAM will give a solution among many other (possible) solutions.

#### B. Approximate Analytical Solutions of the System of (2) Using Homotopy Analysis Method

In this appendix, we indicate how (4) and (5) in this paper are derived. The homotopy analysis method was constructed to determine the solution of (2) In order to solve (B.1) by means of the HAM, we first construct the zeroth-order deformation equation by taking , subject to the following initial conditions: where is an embedding parameter and is the so-called convergence control parameter. When From (B.5) and initial condition (B.3), we get When , (B.2) are equivalent to (B.1), thus it holds the following: Expanding and in Taylor series with respect to the embedding parameter , we have where and will be determined later. Note that the above series contains the convergence control parameter . Assuming that is chosen so properly that the above series is convergent at , we have the solution series as follows: where Equating the like coefficients of the like powers of we have and so on. Solving (B.13) using the initial condition (B.3) and (B.4), we obtain the following result: Adding (B.6), (B.14), (B.7), and (B.15), we get (4) and (5) in the text. Here we can obtain the solution of the equation using only two iterations which leads to the high accuracy.

#### Nomenclature

: | Molar concentration of the native enzyme form (mole/cm^{3}) |

: | Molar concentration of the denatured enzyme form (mole/cm^{3}) |

, , , : | Rate constants of individual reaction (s^{−1}) |

, , : | Modified rate constants (s^{−1}) |

: | Coefficient in the enthalpy balance (s^{−1}) |

: | Bath temperature (K) |

: | Temperature (K) |

: | Time (s). |

#### Acknowledgments

This work was supported by the Council of Scientific and Industrial Research (CSIR no. 01(2442)/10/EMR-II), the Government of India. The authors are thankful to Dr. R. Murali, the Principal, The Madura College, Madurai, and Mr. S. Natanagopal, the Secretary, Madura College Board, Madurai for their encouragement. It is a pleasure to thank the referees for their valuable suggestions.

#### References

- V. Illeova, M. Polakovic, V. Stefuca, P. Acai, and M. Juma, “Experimental modelling of thermal inactivation of urease,”
*Journal of Biotechnology*, vol. 105, no. 3, pp. 235–243, 2003. View at Publisher · View at Google Scholar - H. L. T. Mobley and R. P. Hausinger, “Microbial ureases: significance, regulation, and molecular characterization,”
*Microbiological Reviews*, vol. 53, no. 1, pp. 85–108, 1989. View at Scopus - J. B. Summner, “The isolation and crystallization of the enzyme urease,”
*The Journal of Biological Chemistry*, vol. 69, pp. 435–441, 1926. - N. E. Dixon, C. Gazzola, R. L. Blakeley, and B. Zerner, “Jack Bean urease (EC 3.5.1.5). A metalloenzyme. A simple biological role for nickel?”
*Journal of the American Chemical Society*, vol. 97, no. 14, pp. 4131–4133, 1975. View at Scopus - F. Winquist, I. Lundstrom, and B. Danielsson, “Trace level analysis of mercury using urease in combination with an ammonia gas sensitive semiconductor structure,”
*Analytical Letters*, vol. 21, no. 10, pp. 1801–1816, 1988. View at Scopus - J. C. Polacco and E. A. Havir, “Comparisons of soybean urease isolated from seed and tissue culture,”
*Journal of Biological Chemistry*, vol. 254, no. 5, pp. 1707–1715, 1979. View at Scopus - O. Prakash and G. Bhushan, “Isolation, purification and partial characterisation of urease from seeds of water melon (Citrullus vulgaris),”
*Journal of Plant Biochemistry and Biotechnology*, vol. 6, no. 1, pp. 45–47, 1997. View at Scopus - N. E. Dixon, C. Gazzola, R. L. Blakeley, and B. Zerner, “Jack Bean urease (EC 3.5.1.5). A metalloenzyme. A simple biological role for nickel?”
*Journal of the American Chemical Society*, vol. 97, no. 14, pp. 4131–4133, 1975. View at Scopus - F. Winquist, I. Lundstrom, and B. Danielsson, “Trace level analysis of mercury using urease in combination with an ammonia gas sensitive semiconductor structure,”
*Analytical Letters*, vol. 21, no. 10, pp. 1801–1816, 1988. View at Scopus - M. Hirai, R. Kawai-Hirai, T. Hirai, and T. Ueki, “Structural change of jack bean urease induced by addition of surfactants studied with synchrotron-radiation small-angle X-ray scattering,”
*European Journal of Biochemistry*, vol. 215, no. 1, pp. 55–61, 1993. View at Scopus - R. W. Lencki, J. Arul, and R. J. Neufeld, “Effect of subunit dissociation, denaturation, aggregation, coagulation, and decomposition on enzyme inactivation kinetics: II. Biphasic and grace period behavior,”
*Biotechnology and Bioengineering*, vol. 40, no. 11, pp. 1427–1434, 1992. View at Publisher · View at Google Scholar · View at Scopus - S. Omar and M. Beauregard, “Dissociation and unfolding of jack bean urease studied by fluorescence emission spectroscopy,”
*Journal of Biotechnology*, vol. 39, no. 3, pp. 221–228, 1995. View at Publisher · View at Google Scholar · View at Scopus - S. Liao, “On the homotopy analysis method for nonlinear problems,”
*Applied Mathematics and Computation*, vol. 147, no. 2, pp. 499–513, 2004. View at Publisher · View at Google Scholar · View at Scopus - Y. Liu, “Application research of homotopy analysis method in nonlinear water wave equations,” in
*Proceedings of the 20th International Offshore and Polar Engineering Conference (ISOPE '10)*, pp. 279–282, Beijing, China, June 2010. View at Scopus - Y. Tan and S. Abbasbandy, “Homotopy analysis method for quadratic Riccati differential equation,”
*Communications in Nonlinear Science and Numerical Simulation*, vol. 13, no. 3, pp. 539–546, 2008. View at Publisher · View at Google Scholar · View at Scopus - S. Abbasbandy, “Approximate solution for the nonlinear model of diffusion and reaction in porous catalysts by means of the homotopy analysis method,”
*Chemical Engineering Journal*, vol. 136, no. 2-3, pp. 144–150, 2008. View at Publisher · View at Google Scholar · View at Scopus - S. Abbasbandy, “Homotopy analysis method for heat radiation equations,”
*International Communications in Heat and Mass Transfer*, vol. 34, no. 3, pp. 380–387, 2007. View at Publisher · View at Google Scholar · View at Scopus - Z. Abbas, S. Vahdati, F. Ismail, and A. K. Dizicheh, “Application of homotopy analysis method for linear integro-differential equations,”
*International Mathematical Forum*, vol. 5, no. 5, pp. 237–2249, 2010. *MATLAB 6.1*, The MathWorks Inc., Natick, Mass, USA, 2000, http://www.scilab-enterprises.com/.- S. Liao, “On the homotopy analysis method for nonlinear problems,”
*Applied Mathematics and Computation*, vol. 147, no. 2, pp. 499–513, 2004. View at Publisher · View at Google Scholar · View at Scopus - H. Jafari, C. Chun, and S. M. Saeidy, “Analytical solution for nonlinear gas dynamic homotopy analysis method,”
*Applications and Applied Mathematics*, vol. 4, pp. 149–154, 2009. - S. J. Liao,
*Beyond Perturbation: Introduction to the Homotopy Analysis Method*, Chapman and Hall, CRC Press, Boca Raton, Fla, USA, 1st edition, 2003.