In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components

Table 1

Effect of different formulations of basic culture media on seedling growth parameters on in vitro germination of Handroanthus impetiginosus after 30-day culture.

	MSG^1	WPM^2
Shoot length (mm)	59.6 ± 18.0 a	56.0 ± 20.8 a
Shoot fresh weight (mg)	487.3 ± 108.2 a	411.6 ± 91.4a
Shoot dry weight (mg)	66.9 ± 15.8 a	96.5 ± 22.8 a
Root length (mm)	131.4 ± 26.4 a	109.7 ± 33.4 b
Root fresh weight (mg)	211.8 ± 30.9 a	201.9 ± 17.5 a
Root dry weight (mg)	40.5 ± 13.3 a	34.1 ± 7.0 a
Root surface area (mg)^3	140.8 ± 75.5 a	142.3 ± 73.3 a
Hyperhydric shoots (%)	0	0

¹MSG: Murashige and Skoog [9] salts with Gamborg vitamins [18] medium. ²WPM: Woody Plant Medium [10]. ³Expressed as mg of saturated solution Ca(NO₃)₂ according to [19]. Different letters in the same row indicate significant differences between treatments by Tukey’s multiple range test (𝑃≤0.05). Data are the averages of 3 replications of 25 seeds.