In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components

Table 2

Effect of different concentrations of benzylaminopurine (BA) on in vitro shoot multiplication of Handroanthus impetiginosus on MSG and WPM basic media after 30-day culture.

Media	BA (μM)	MR^1	Shoots length (mm)	Internodes length (mm)	Shoots number^2	Callus formation^3	Hh^4 (%)	Survival (%)
MSG	0	1.68 ± 0.61 a	19.06 ± 5.02 a	5.77 ± 2.29 bc	1.26 ± 0.22 ab	−	0	98
WPM	0	1.66 ± 0.40 a	21.25 ± 6.58 a	6.78 ± 1.96 ab	1.00 ± 0.00 b	−	0	100
MSG	5	2.51 ± 0.80 a	17.06 ± 4.66 ab	5.08 ± 1.98 c	1.54 ± 0.26 a	+	3	86.5
WPM	5	1.58 ± 0.48 a	18.92 ± 4.67 a	7.72 ± 2.37 a	1.23 ± 0.26 ab	−	0	100
MSG	10	1.52 ± 0.53 a	13.69 ± 6.66 b	4.95 ± 1.74 c	1.20 ± 0.19 ab	++	5	83.8
WPM	10	2.01 ± 0.50 a	17.02 ± 6.47 ab	5.95 ± 1.36 bc	1.47 ± 0.38 a	+	2	98

MSG: Murashige and Skoog [9] salts with Gamborg’s vitamins [18] medium. WPM: Woody Plant Medium [10]. ¹Multiplication rate: number of usable shoots obtained per initial shoot. ²Initial and adventitious shoots per explant. ³Callus absence (−) or presence (+). ⁴Hh: percentage of hyperhydric shoots in scale 1 to 5. Different letters in the same column indicate significant differences between treatments by Tukey’s multiple range test (𝑃≤0.05). Data are the averages of 2 replications of at least 56 shoots each.