In Vitro Propagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components
Table 5
Effect of indole butyric acid (IBA) concentration and different formulations of basic culture media (1/2 WPM and 1/2 MSG) on shoot development parameters of in vitro rooting of Handroanthus impetiginosus with three days of auxin pulse induction, after 40-day culture.
Medium
IBA (μM)
Leaf number
Shoot length
Fresh shoot weight (mg)
Dry shoot weight (mg)
1/2 WPM
0
6.49 ± 1.01 ab
20.28 ± 2.02 b
47.17 ± 3.69 abc
9.98 ± 1.09 abc
10
4.15 ± 0.88 cd
24.44 ± 2.20 ab
37.64 ± 3.01 bcd
8.35 ± 1.27 abc
20
4.06 ± 0.91 d
21.92 ± 2.89 ab
31.21 ± 4.05 cd
6.81 ± 1.53 abcd
30
5.32 ± 0.78 abcd
23.89 ± 2.51 ab
47.09 ± 6.16 abc
10.47 ± 2.03 ab
40
4.45 ± 0.99 bcd
20.75 ± 1.20 b
29.60 ± 4.12 d
5.92 ± 1.28 cd
50
4.04 ± 1.11 d
21.56 ± 1.86 ab
28.13 ± 5.76 d
4.75 ± 2.35 d
1/2 MSG
0
5.52 ± 1.06 abcd
21.17 ± 1.75 b
42.65 ± 4.68 abcd
7.39 ± 1.37 abcd
10
4.55 ± 0.90 bcd
22.14 ± 2.59 ab
36.36 ± 6.39 bcd
6.78 ± 2.98 abcd
20
6.64 ± 1.28 a
23.50 ± 2.62 ab
54.62 ± 4.66 ab
6.32 ± 0.27 bcd
30
5.35 ± 1.22 abcd
24.24 ± 2.60 ab
51.36 ± 5.43 ab
6.79 ± 1.63 abcd
40
6.16 ± 1.85 ab
29.06 ± 4.36 a
58.49 ± 9.48 a
5.92 ± 0.61 cd
50
6.05 ± 1.46 abc
24.78 ± 2.78 ab
61.94 ± 6.27 a
11.13 ± 2.16 a
1/2 WPM: Woody Plant Medium [10] half concentration. 1/2 MSG: Murashige and Skoog [9] salts at half concentration with Gamborg vitamins [18]. Different letters in the same column indicate significant differences between treatments by Tukey’s multiple range test (). Data are the average of 3 replications of 20 shoots.
