UHPLC/MS-MS Analysis of Six Neonicotinoids in Honey by Modified QuEChERS: Method Development, Validation, and Uncertainty Measurement

Table 2

Method validation results.

Compound	𝑅𝑡 (min)	Linearity range; (μg/L)	𝑟^2	LOQ (µg/kg)	Recovery %; (10 μg/kg)^†	Recovery %; (40 μg/kg)^†
Thiamethoxam	2.63	1–50	0.999	0.50	101 ± 11	100 ± 12
Nitenpyram	2.82	1–50	0.998	4.00	75 ± 20	97 ± 9
Imidacloprid	3.30	1–50	0.998	2.80	114 ± 3	109 ± 7
Clothianidin	3.39	1–50	0.999	3.20	111 ± 8	105 ± 9
Acetamiprid	3.89	1–50	0.995	0.12	107 ± 5	105 ± 6
Thiacloprid	4.46	1–50	0.997	0.10	89 ± 6	92 ± 2

†Mean value of six determinations; relative standard deviations (precision) in parenthesis.