Effects of Subminimum Inhibitory Concentrations of Antibiotics on the Pasteurella multocida Proteome: A Systems Approach

Table 3

Differential P. multocida protein expression in response to a quarter-MIC AMX, CTC, and ENR in different GO categories.

Accession	GO Slims	∑Xcorr
		Pm70	AMX	CTC	ENR
Glycolysis/Gluconeogenesis
15603407	Phosphoenolpyruvate carboxykinase, PckA	264.2	ns	120	112.9
15603725	Phosphoglycerate kinase, Pgk	218.1	ns	170.2	161
15603726	Fructose-bisphosphate aldolase, FbaA	504.9	566.6	450.1	436.8
15602789	Glyceraldehyde-3-phosphate dehydrogenase, GapA	253.6	ns	125.5	70.1
15603371	Phosphoglyceromutase, GpmA	98.3	124.9	130.3	ns
15602518	Pyruvate kinase, PykA	28.6	51.4	54.9	ns
15602688	Fumarate hydratase, FumC	42.2	80.5	19.1	ns
15602146	SucD	bdt	7.8	ns	ns
Response to stress
15602972	Chaperonin GroEL	148.6	246.8	ns	177.1
15602971	Co-chaperonin GroES	23	42.7	56.3	56.6
15602601	DnaK	97.6	132.7	ns	72.6
15602303	FtsH	21.9	7.9	4.7	3.9
Peptidoglycon biosynthesis and cell envelope permeability
15603596	D-fructose-6-phosphate amidotransferase, GlmS	bdt	31.3	12.4	6.8
15603671	GlmU	8.5	20.2	ns	ns
15602548	NagC	14.4	ns	5.3	5
15602651	Hypothetical protein PM0786, OmpA	262.8	ns	172.5	76
15602419	Lpp	7.2	ns	18.7	ns
Purine metabolism
15602149	Adenylate kinase, Adk	2.8	22.3	42.2	11.3
15602803	Adenylosuccinate synthetase, PurA	2.4	ns	11.2	19.2
15602109	Ribose-phosphate pyrophosphokinase, PrsA	bdt	9.2	8.4	ns
RNA metabolism
15603255	DNA-directed RNA polymerase alpha subunit, RpoA	32.8	76.1	51.9	ns
15603602	DNA-directed RNA polymerase beta subunit, RpoB	17	34.5	ns	ns
15603601	DNA-directed RNA polymerase beta~ subunit, RpoC	56.2	85.2	ns	ns
15602786	DNA-directed RNA polymerase omega subunit, RpoZ	bdt	11	ns	9.6
15602119	Lrp	8.7	ns	25.1	ns
15603209	DeoR	bdt	10.6	ns	ns
15603785	Transcription termination factor Rho	42.4	15.9	20.5	21.3
Xenobiotic metabolism
15601866	SodA	153.5	ns	66	85.4
15601897	HktE/KatE	26.1	ns	3	6.1
Lipopolysaccharide biosynthesis
15603862	Lipid-A-disaccharide synthase, LpxB	bdt	12.1	ns	ns
15602423	2-dehydro-3-deoxyphosphooctonate aldolase, KdsA	9.2	23.2	ns	ns
15602749	RfaE	bdt	12.9	ns	ns
15603154	GalU	3.7	18.7	ns	ns
Cell division
15603384	FtsY	bdt	8.2	ns	ns
15602012	Cell division protein FtsZ	11.5	22.1	ns	ns
Protein translation
15603222	Elongation factor Tu	875.7	ns	936	ns
15602977	DeaD	4.7	ns	13.3	ns
15602168	Methionyl-tRNA synthetase	2.4	ns	11.5	ns
DNA repair
15603682	Recombinase A, RecA	bdt	ns	ns	19.5

AMX: amoxicillin; CTC: chlortetracycline; ENR: enrofloxacin.
Pm70: P. multocida cultured without sub-MICs.
bdt: below detectable threshold.
ns: differential expression not significant compared to Pm70.