The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in Hybrids
Figure 3
Location of spot 200. Schematic of the region of chromosome 11 containing the restriction enzyme sites located in the region to the transcription start site of the non-protein coding transcript (Os11g0417300). The DNA fragments were digested at the NotI (N) and MspI/HpaII (M) sites and fractionated by one dimensional electrophoresis. Next, the DNA fragments that were digested at the BamHI (B) sites were fractionated by two dimensional electrophoresis, which allowed detection of the B-N fragment as an RLGS spot. Spot 200 corresponds to the fragment between the N and B sites. The N and M sites were identified in the parental Nipponbare and Kasalath, and in both reciprocal hybrids. The B site was only absent in Kasalath, resulting in the absence of spot 200 in the RLGS pattern.
