The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in Hybrids
Figure 4
Expression analysis of the nearest gene to the methylated site. (a) RT-PCR showed that a non-protein coding transcript (Os11g0417300) was expressed in leaf blade and sheath of Nipponbare, Kasalath, NKF1, and KNF1 plants. (b) Sequence analysis of the RT-PCR products of the expressed Os11g0417300 allele. The single nucleotide polymorphism between Nipponbare (C) and Kasalath (T) is indicated in the RT-PCR products by arrowheads. Specific expression of the Nipponbare allele was confirmed by detection of base C in both NK5 and KN5 plants. (c) Sequence analysis of RT-PCR products of the expressed Os01g0327900 allele. The single nucleotide polymorphism in RT-PCR products between Nipponbare (C) and Kasalath (A) is indicated by arrowheads. Specific expression of the Kasalath allele was confirmed by detection of base A in both NK7 and KN10 plants.
