International Journal of Genomics / 2009 / Article / Tab 1 / Research Article
Constructing Physical and Genomic Maps for Puccinia striiformis f. sp. tritici , the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence of P. graminis f. sp. tritici , the Wheat Stem Rust Pathogen Table 1 Primer sequences of Pst genes and amplification of same BAC clones . The table shows sequences and annealing temperatures (Tm) of primers based on Pst EST sequences used in PCR screening the Pst BAC library to determine the physical relationships of genes in pairs identified through BLAST search comparison with the Pgt whole genome sequence. The presence and absence of both genes of a pair in the same BAC clone are indicated by “Y” and “N”, respectively.
Gene pair EST clone Forward (
) Reverse (
) Tm
In same BAC clone 1 PST78SP60L20C CTGGTAATGGAGGTGGAACT CTGGGGTAGGTAAGAAGGTC 53.8/53.1 Y PST78SP15C21F AAGCCCCTCCAGTAGAAC CACATCCCAGACGGTAAAT 51.0/52.6 2 PST78Ha6E1 GACTGGTGTCATTGCTGAA TGGAGGAAGATACTTGGAGA 51.2/51.8 N PST78SP15K17F CCGAAATACCCCAGAACT GTCAACGATGAACAGAAGAG 52.0/49.0 3 PST78HaC443 CCTCGTCTTCACCTTCATTA TGTTTAGTTCTTGTCAGCGT 52.6/50.7 Y PST78SP19J9F AGAATCAGCACGAAAGGG TTGAGGTAGGTTGGACGG 52.0/53.0 4 PSTCY32GTC198 ACATTATTCGCTTCCCTTTC TGAGTTTTGTGGTATCGGTC 54.0/53.0 Y PST78SP17H12F CACAACTACCAGACCCAG CGTAGGAAGTCGGATAAG 47.0/47.0 5 PST78SP65I3C CAGGAGGAAAACAACCATAA ATCGTACTAAGCACCCATCA 52.8/52.9 N PST78Ha9B6 GTTTGATTGAGCGGGATTC CCCATTCATCTGCCTGTTT 55.0/55.0 6 PSTCY32GTC60 GGAACTACCAGGACTACCC CACCCATACTTCTGAGGC 50.5/50.0 Y PST78Ha10O15 GGGTAGTGCTCCCAAAC TTCTGCCGTCAAAGTGT 49.7/48.6 7 PST78HaC375 CAGTCGTCCTCAAAATCCTA TCCCCAGCACTATTCCTTAT 52.6/54.1 N PST78Ha10O15 GGGTAGTGCTCCCAAAC TTCTGCCGTCAAAGTGT 49.7/48.6 8 PSTCY32GT1614 ACACGTAAGGACAGCAGAA GGATAACAAGGAAGGGAGA 50.7/51.2 Y PST78Ha14M14 TCAGAAACAAGACCCACC CCCACTTCACTACCCATTA 50.0/50.3 9 PST78SP6D14F GAATCCACTCCATCCCACT TACGGCTCCGAGAACGAC 53.7/56.4 N PST78SP14N13F AACACCAGCAGCACAACT TGTAGCCCTAACCTTCGT 50.8/50.1 10 PSTCY32GTC331 CGTCCTTGGCTGAATCTC CGGCTACACCACGAACAT 53.0/54.3 N PST78SP20d10F ACACCAGCATCGCAAAAC GAACGAGCGTGAGGGAGA 54.4/56.1 11 PSTCY32GTC164 TGCCAGTCCGAGTATCAAGA GTAGCAGATTCCGAGTCCAA 56.2/55.1 Y PST78SP11F24F CGGAGGAACAGCTACAAAAG GGAGAAGGGATAACCCAGAC 55.3/55.0 12 PSTCY32GTC285 CAGCCTCACTAACAACATCG TAATAGGACAGGAGCAGACA 53.8/50.3 N PST78SP18M12F AACCCTGCCACAATGATGAC GGACGGGGAAACAATAGAGC 57.7/58.7 13 PSTCY32GT429 GGCTGCTGAATATGACCGAA GCCTGCCACATCACTACCTG 58.5/58.5 N PST78SPC50F GAGGCGTCTGGTGGGATAAG CCGTAAAGAGGTTTCCGAGATGAT 59.4/63.1 14 PSTCY32GTC220 CAACTGACACCGCTGAAA CGCCTTCTTGGAATGACT 52.5/52.8 Y PST78SP66B11CF ATGATGGCGGATAGAACA GCTACCCGACCTCACTTT 51/51.9 15 PST78SP10f11F CCGCAGTCGCTGTATGTA TGTATCCAACTTGCCCAC 53.0/51.3 Y PSTCY32GT407 ACGACTGCTTCTGCTTCA ATCCTCGCCATTCTTCTT 51.3/51.5 16 PST78SP3H2F CGAAGACCAGCAAAATGT CACGGAGATGGAAAGAAT 51.0/49.6 Y PST78Ha8F3 ACTTTACCAAGATGACCC GTGAAGTAATCCCAAACC 45.9/46.5 17 PSTCY32GT1071 ACCCTGGAAAAGGCGAAAT GCGATGATGCCCGATGTA 58.7/57.9 Y PST78SPC194F GACGCCAGTCGTAGCACA GGGATTGAGGGACGCATA 55.3/56.0 18 PST78SP65M2C AATCTTATGTTCAAGTTCGGTT TTCGTTTCTGTTAATACTCCTA 53.2/49.9 N PSTCY32GT5910 ACCAAACGAAAGAACAAG TTCACTCTACCAACAGCA 47.6/45.9 19 PSTCY32GT2267 AAGACCACCTCGCTCAAC GGAAATACGTCCGCAAAT 52.3/53.2 N PSTCY32GT1090 CGACGACTACCACGACAT ACGATAGCTTGCCATCAC 51.1/50.8 20 PST78Ha1507 GCCAATCAAGGATGCTCT GAAGTTCCGCCGTAGTGT 52.6/52.8 Y PST78HaC376 CCCTACTACGACCTCCA CTGCTTCTACCCATCCA 47.3/48.9 21 PSTCY32GTC279 CCAAACAACCAAACGACGAA GACCGAAAGCGGGTGAATAG 59.3/59.7 Y PSTCY32GT1972 CTCAAGATACATCGTCCC AAGTTGGTCAGGCAGTTC 46.9/49.5
